Secretory Profiles and Wound Healing Effects of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

被引：157

作者：

Yoon, Byung Sun ^{[1
,2
]}

Moon, Jai-Hee ^{[1
]}

Jun, Eun Kyoung ^{[1
,4
]}

Kim, Jonggun ^{[3
]}

Maeng, Isaac ^{[1
]}

Kim, Jun Sung ^{[1
]}

Lee, Jung Han ^{[1
,4
]}

Baik, Cheong Soon ^{[5
]}

Kim, Aeree ^{[6
]}

Cho, Kyoung Shik ^{[4
]}

Lee, Jang Ho ^{[4
]}

Lee, Hwang Heui ^{[4
,7
]}

Whang, Kwang Youn ^{[3
]}

You, Seungkwon ^{[1
]}

机构：

[1] Korea Univ, Lab Cell Funct Regulat, Coll Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Korea Univ, Inst Life Sci & Nat Resources, Seoul 136701, South Korea

[3] Korea Univ, Div Biotechnol, Coll Life Sci & Biotechnol, Seoul 136701, South Korea

[4] Stemmedience Corp, Div Stem Cell, Res Inst, Seoul, South Korea

[5] SamKwang Med Labs, Seoul, South Korea

[6] Korea Univ, Guro Hosp, Dept Pathol, Coll Med, Seoul 136701, South Korea

[7] ChungDam NB Clin, Seoul, South Korea

来源：

STEM CELLS AND DEVELOPMENT | 2010年 / 19卷 / 06期

关键词：

UMBILICAL-CORD BLOOD; HEPATOCYTE GROWTH-FACTOR; BONE-MARROW; STROMAL CELLS; IN-VITRO; EXPRESSION; DIFFERENTIATION; ADULT; CYTOKINES; GENES;

D O I：

10.1089/scd.2009.0138

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-beta, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-beta/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-beta/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.

引用

页码：887 / 902

页数：16

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