Elimination of False-Positive Results in a Luminex Assay for Pneumococcal Antibodies

被引：26

作者：

Pickering, Jerry W. ^{[1
]}

Larson, Matthew T. ^{[1
]}

Martins, Thomas B. ^{[1
]}

Copple, Susan S. ^{[2
]}

Hill, Harry R. ^{[1
,3
]}

机构：

[1] ARUP Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA

[2] ARUP Labs, Salt Lake City, UT USA

[3] Univ Utah, Sch Med, Dept Pathol Pediat & Med, Salt Lake City, UT USA

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2010年 / 17卷 / 01期

关键词：

FLUORESCENT MICROSPHERE IMMUNOASSAY; LINKED-IMMUNOSORBENT-ASSAY; SIMULTANEOUS QUANTITATION; CAPSULAR POLYSACCHARIDES; NEUTRALIZING EPITOPES; MULTIPLEX IMMUNOASSAY; BORDETELLA-PERTUSSIS; SERUM-ALBUMIN; VALIDATION; DIPHTHERIA;

D O I：

10.1128/CVI.00329-09

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.

引用

页码：185 / 189

页数：5

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