The temperature activated HtrA protease from pathogen Chlamydia trachomatis acts as both a chaperone and protease at 37 °C

被引：47

作者：

Huston, Wilhelmina M. ^{[1
]}

Swedberg, Joaquim E.

Harris, Jonathan M.

Walsh, Terence P.

Mathews, Sarah A.

Timms, Peter

机构：

[1] Queensland Univ Technol, Fac Sci, Inst Hlth & Biomed Innovat, Brisbane, Qld, Australia

[2] Queensland Univ Technol, Fac Sci, Sch Life Sci, Brisbane, Qld, Australia

来源：

FEBS LETTERS | 2007年 / 581卷 / 18期

基金：

英国医学研究理事会;

关键词：

HtrA; serine protease; chaperone; Chlamydia;

D O I：

10.1016/j.febslet.2007.06.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterization of the protease, HtrA, from pathogen Chlamydia trachomatis is presented. The purified recombinant protein was a serine endoprotease, specific for unfolded proteins, and temperature activated above 34 degrees C. Chaperone activity was observed, although this appeared target-dependent. Inactive protease (S247A) was able to chaperone insulin B-chain, irrespective of temperature, but at 30 degrees C only HtrA and not S247A displayed significant chaperone activity for alpha-lactalbumin. These data demonstrate that chaperone activity may involve functional protease domain and that C. trachomatis HtrA functions as both a chaperone and protease at 37 degrees C. These properties are consistent with the developmental cycle of this obligate intracellular bacterium. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3382 / 3386

页数：5

共 31 条

[1] The chlamydial developmental cycle [J].

AbdelRahman, YM ;

Belland, RJ .

FEMS MICROBIOLOGY REVIEWS, 2005, 29 (05) :949-959

[2] EVALUATION OF SECONDARY STRUCTURE OF PROTEINS FROM UV CIRCULAR-DICHROISM SPECTRA USING AN UNSUPERVISED LEARNING NEURAL-NETWORK [J].

ANDRADE, MA ;

CHACON, P ;

MERELO, JJ ;

MORAN, F .

PROTEIN ENGINEERING, 1993, 6 (04) :383-390

[3] Role of HtrA in surface protein expression and biofilm formation by Streptococcus mutans [J].

Biswas, S ;

Biswas, I .

INFECTION AND IMMUNITY, 2005, 73 (10) :6923-6934

[4] Mutation R120G in αB-crystallin, which is linked to a desmin-related myopathy, results in an irregular structure and defective chaperone-like function [J].

Bova, MP ;

Yaron, O ;

Huang, QL ;

Ding, LL ;

Haley, DA ;

Stewart, PL ;

Horwitz, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6137-6142

[5] The HtrA protease of campylobacter jejuni is required for heat and oxygen tolerance and for optimal interaction with human epithelial cells [J].

Brondsted, L ;

Andersen, MT ;

Parker, M ;

Jorgensen, K ;

Ingmer, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (06) :3205-3212

[6] THE ACYLATED PRECURSOR FORM OF THE COLICIN-A LYSIS PROTEIN IS A NATURAL SUBSTRATE OF THE DEGP PROTEASE [J].

CAVARD, D ;

LAZDUNSKI, C ;

HOWARD, SP .

JOURNAL OF BACTERIOLOGY, 1989, 171 (11) :6316-6322

[7] Regulation of HAX-1 anti-apoptotic protein by Omi/HtrA2 protease during cell death [J].

Cilenti, L ;

Soundarapandian, MM ;

Kyriazis, GA ;

Stratico, V ;

Singh, S ;

Gupta, S ;

Bonventre, JV ;

Alnemri, ES ;

Zervos, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (48) :50295-50301

[8] Characterization of a novel and specific inhibitor for the pro-apoptotic protease Omi/HtrA2 [J].

Cilenti, L ;

Lee, Y ;

Hess, S ;

Srinivasula, S ;

Park, KM ;

Junqueira, D ;

Davis, H ;

Bonventre, JV ;

Alnemri, ES ;

Zervos, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11489-11494

[9] The HtrA family of proteases: Implications for protein composition and cell fate [J].

Clausen, T ;

Southan, C ;

Ehrmann, M .

MOLECULAR CELL, 2002, 10 (03) :443-455

[10] Role of the htrA gene in Klebsiella pneumoniae virulence [J].

Cortés, G ;

de Astorza, B ;

Benedí, VJ ;

Albertí, S .

INFECTION AND IMMUNITY, 2002, 70 (09) :4772-4776

← 1 2 3 4 →