Cloning and developmental regulation of tissue inhibitor of metalloproteinases-3 (TIMP3) in Xenopus laevis early embryos

被引：5

作者：

Yang, MZ

Kurkinen, M ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI 48202 USA

[2] Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48202 USA

来源：

GENE | 1998年 / 211卷 / 01期

关键词：

matrix metalloproteinase inhibitors development; Xenopus;

D O I：

10.1016/S0378-1119(98)00077-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned a cDNA encoding tissue inhibitor of metalloproteinases-3 (TIMP3) from the frog Xenopus laevis. Similar to TIMP3 from other species, Xenopus TIMP3 has 188 residues including 12 conserved cysteines and Asn(184), a putative site for N-linked sugars. Xenopus TIMP3 is 84% identical with human TIMP3. As shown by Northern blotting and RT-PCR, Xenopus TIMP3 mRNA is maternally inherited in eggs and midblastula (stage 8) embryos, downregulated in gastrula and then upregulated in neurula and pretailbud embryos. In select adult tissues, TIMP3 mRNA is present in heart, muscle, liver, skin, intestine and ovaries. These results suggest that TIMP3 is involved in the regulation of expression of matrix metalloproteinases in Xenopus early development and adult tissue remodeling. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 100

页数：6