Extraction and ultra-performance liquid chromatography of hydrophilic an lipophilic bioactive components in a Chinese herb Radix Salviae Miltiorrhizae

被引：67
作者：
Liu, Mei
Li, Yongguo
Chou, Guixin
Cheng, Xuemei
Zhang, Mian
Wang, Zhengtao
机构：
[1] Shanghai Univ Tradit Chinese Med, Minist Educ, Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China
[2] China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210038, Peoples R China
[3] Shanghai R&D Ctr Standardizat Chinese Med, Shanghai 201203, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1157卷 / 1-2期
关键词：
Radix Salviae Miltiorrhizae; fingerprinting; salvianolic acids; tanshinone; UPLC; HPLC;
D O I：
10.1016/j.chroma.2007.05.018
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An ultra-performance liquid chromatography (UPLC) method has been developed and validated for the quality evaluation of a Chinese herb Radix Salviae Miltiorrhizae (Danshen root) by chemical fingerprinting analysis. Meanwhile a novel sample preparation procedure has been designed which can simultaneously extract both the hydrophilic and lipophilic components for a single run LC system. Comparing to the conventional HPLC method. UPLC showed many advantages including reduced run time, less solvent consumption and increased peak capacities. Using the established method. 20 target components were detected based on the retention times and on-line UV spectra by referencing to the available standards and reported data. Eleven crude drug samples from different sources were evaluated using the UPLC fingerprints. The developed method proved practicable and reliable for quality control of herbal products with multivalent components in a complicated matrix. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：51 / 55
页数：5
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