Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

被引：75
作者：
Kim, Tae-Il [1 ,2 ]
Hwang, Byunghee [1 ,2 ]
Lee, Boeun [3 ]
Bae, Jeehyeon [4 ]
Kim, Youngmi [1 ,2 ]
机构：
[1] Kyung Hee Univ, Dept Chem, 26 Kyungheedae Ro, Seoul 02447, South Korea
[2] Kyung Hee Univ, Res Inst Basic Sci, 26 Kyungheedae Ro, Seoul 02447, South Korea
[3] Chung Ang Univ, Dept Life Sci, 84 Heukseok Ro, Seoul 06974, South Korea
[4] Chung Ang Univ, Sch Pharm, 84 Heukseok Ro, Seoul 06974, South Korea
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2018年 / 140卷 / 37期
基金：
新加坡国家研究基金会;
关键词：
HYPOBROMOUS ACID; FLUORESCENT-PROBE; LIVING CELLS; HYPOCHLOROUS ACID; CANCER-CELLS; MOLECULAR-MECHANISMS; GLUCOSE DEPRIVATION; OXIDATIVE STRESS; H2O2; MEDIATE; BODIPY DYES;
D O I：
10.1021/jacs.8b07073
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (>= 1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.
引用
收藏
页码：11771 / 11776
页数：6
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