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Characterization on hydrogen production performance of a newly isolated Clostridium beijerinckii YA001 using xylose
被引:51
作者:
An, Dan
[1
]
Li, Qing
[1
]
Wang, Xueqing
[1
]
Yang, Honghui
[1
]
Guo, Liejin
[1
]
机构:
[1] Xi An Jiao Tong Univ, State Key Lab Multiphase Flow Power Engn, Xian 710049, Peoples R China
基金:
中国国家自然科学基金;
关键词:
Bioconversion;
Biogas;
Batch processing;
Fermentation;
Hydrogen production;
Xylose utilization;
BIOHYDROGEN PRODUCTION;
STATISTICAL OPTIMIZATION;
DARK FERMENTATION;
SUBSTRATE CONCENTRATION;
PH;
TEMPERATURE;
HYDROLYSATE;
PARAMETERS;
VARIABLES;
STRAIN;
D O I:
10.1016/j.ijhydene.2014.10.014
中图分类号:
O64 [物理化学(理论化学)、化学物理学];
学科分类号:
070304 ;
081704 ;
摘要:
A mesophilic bacterium was isolated from cow manure, and identified as Clostridium beijerinckii YA001 based on 16S rRNA gene sequence calculation using MEGA 5.0 and biochemical tests. This strain showed the ability to digest a wide range of carbon and nitrogen sources for hydrogen production, and it showed high hydrogen production performance using xylose as substrate. The optimum parameters for bio-hydrogen production in batch tests were pH 8.0, 1% substrate concentration, 40 degrees C and yeast extract as nitrogen source. The maximum hydrogen yield and the hydrogen production rate were obtained at 2.31 mol/mol xylose and 311.3 mL H-2/(Lh), respectively. These results indicate that C. beijerinckii YA001 is an ideal candidate for fermentative hydrogen production. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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页码:19928 / 19936
页数:9
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