Characterization on hydrogen production performance of a newly isolated Clostridium beijerinckii YA001 using xylose

被引:51
作者
An, Dan [1 ]
Li, Qing [1 ]
Wang, Xueqing [1 ]
Yang, Honghui [1 ]
Guo, Liejin [1 ]
机构
[1] Xi An Jiao Tong Univ, State Key Lab Multiphase Flow Power Engn, Xian 710049, Peoples R China
基金
中国国家自然科学基金;
关键词
Bioconversion; Biogas; Batch processing; Fermentation; Hydrogen production; Xylose utilization; BIOHYDROGEN PRODUCTION; STATISTICAL OPTIMIZATION; DARK FERMENTATION; SUBSTRATE CONCENTRATION; PH; TEMPERATURE; HYDROLYSATE; PARAMETERS; VARIABLES; STRAIN;
D O I
10.1016/j.ijhydene.2014.10.014
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A mesophilic bacterium was isolated from cow manure, and identified as Clostridium beijerinckii YA001 based on 16S rRNA gene sequence calculation using MEGA 5.0 and biochemical tests. This strain showed the ability to digest a wide range of carbon and nitrogen sources for hydrogen production, and it showed high hydrogen production performance using xylose as substrate. The optimum parameters for bio-hydrogen production in batch tests were pH 8.0, 1% substrate concentration, 40 degrees C and yeast extract as nitrogen source. The maximum hydrogen yield and the hydrogen production rate were obtained at 2.31 mol/mol xylose and 311.3 mL H-2/(Lh), respectively. These results indicate that C. beijerinckii YA001 is an ideal candidate for fermentative hydrogen production. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:19928 / 19936
页数:9
相关论文
共 45 条
[1]   Effects of temperature and substrate concentration on biological hydrogen production from starch [J].
Akutsu, Yohei ;
Li, Yu-You ;
Harada, Hideki ;
Yu, Han-Qing .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2009, 34 (06) :2558-2566
[2]   Regulation of hydrogen metabolism in Butyribacterium methylotrophicum by substrate and pH [J].
Annous, BA ;
Shieh, JS ;
Shen, GJ ;
Jain, MK ;
Zeikus, JG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 45 (06) :804-810
[3]   The effect of nutrient limitation on hydrogen production by batch cultures of Escherichia coli [J].
Bisaillon, Ariane ;
Turcot, Jonathan ;
Hallenbeck, Patrick C. .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2006, 31 (11) :1504-1508
[4]   Fermentative hydrogen production by a new mesophilic bacterium Clostridium sp 6A-5 isolated from the sludge of a sugar mill [J].
Cai, Jinling ;
Wu, Qi ;
Wang, Guangce ;
Deng, Chaobin .
RENEWABLE ENERGY, 2013, 59 :202-209
[5]   Statistical optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16 [J].
Cao, Guang-li ;
Ren, Nan-qi ;
Wang, Ai-jie ;
Guo, Wan-qian ;
Yao, Jing ;
Feng, Yu-jie ;
Zhao, Qing-liang .
BIORESOURCE TECHNOLOGY, 2010, 101 (06) :2053-2058
[6]   Statistical optimization of factors affecting biohydrogen production from xylose fermentation using inhibited mixed anaerobic cultures [J].
Chaganti, Subba Rao ;
Kim, Dong-Hoon ;
Lalman, Jerald A. ;
Shewa, Wudneh Ayele .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2012, 37 (16) :11710-11718
[7]   Feasibility studies on the fermentative hydrogen production by recombinant Escherichia coli BL-21 [J].
Chittibabu, G ;
Nath, K ;
Das, D .
PROCESS BIOCHEMISTRY, 2006, 41 (03) :682-688
[8]   Optimization of initial substrate and pH levels for germination of sporing hydrogen-producing anaerobes in cow dung compost [J].
Fan, YT ;
Li, CL ;
Lay, JJ ;
Hou, HW ;
Zhang, GS .
BIORESOURCE TECHNOLOGY, 2004, 91 (02) :189-193
[9]  
Fang HHP, 2002, APPL MICROBIOL BIOT, V58, P112
[10]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x