Sensitive chemiluminescence assay for risperidone in pharmaceutical preparations

A sensitive chemiluminescence (CL) method to determine the antipsychotic risperidone (RSP) is proposed, based on the catalytic effect of risperidone on the CL reaction between luminol and hydrogen peroxide in flow system. The increment of CL intensity was correlated with risperidone concentration in the range of 10 pg ml(-1) to 1.0 ng ml(-1) with a relative standard deviation of less than 5.0% (n = 5); and a limit of detection of 4 pg ml(-1) (3sigma). At a flow rate of 2.0 ml min(-1), the flow injection CL method exhibited both high sensitivity and excellent selectivity giving a throughput of 120 samples per hour. The proposed method was applied successfully to the determination of risperidone in pharmaceutical preparations. (C) 2004 Elsevier B.V. All rights reserved.