Biosensor based on an oxygen reducing bilirubin oxidase electrode

被引：4
|
作者：
Goebel, G. [1 ]
Dietz, T. [1 ]
Lisdat, F. [1 ]
机构：
[1] Univ Appl Sci Wildau, D-15745 Wildau, Germany
来源：
PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE | 2009年 / 1卷 / 01期
关键词：
enzyme sensor; bilirubin oxidase; carbon nanotubes; membran-free; mediator-less; CARBON ELECTRODES; REDUCTION; DIOXYGEN;
D O I：
10.1016/j.proche.2009.07.068
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An oxygen reducing electrode made of bilirubin oxidase and multi-walled carbon nanotubes (BOD-MWCNT-Au electrode) is coupled to enzymes catalysing oxygen-consuming reactions such as glucose oxidase (GOD) to result in a membrane-free bienzyme electrode. The feasibility of such a molecularly assembled system stabilized by covalent linkage has been demonstrated. The electrochemical characterisation of the bienzyme electrode reveals sensitivity to the enzyme substrate. The results indicate that the BOD-electrode provides a suitable platform for sensing analytes for which oxidases of high activity are available.
引用
收藏
页码：273 / 276
页数：4
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