Modulation by Polypodium leucotomos extract of cytokine patterns in experimental trichomoniasis model

被引:14
作者
Nogal-Ruiz, JJ [1 ]
Gómez-Barrio, A [1 ]
Escario, JA [1 ]
Martínez-Fernández, AR [1 ]
机构
[1] Univ Complutense, Fac Farm, Dept Parasitol, Madrid 28040, Spain
关键词
Trichomonas vaginalis; Polypodium leucotomos; Anapsos (R); experimental trichomoniasis model; immunomodulation; IFN-gamma; IL-4;
D O I
10.1051/parasite/2003101p73
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The immunomodulating effects of Anapsos((R)), an aqueous hydrosoluble extract obtained from the rhizomes of the fern Polypodium leucotomos, on both pathogenicity and cytokine levels in serum (IFN-gamma/IL-4) were assayed in a Trichomonas vaginalis experimental model (BALB/c mice infected with 10(7) trichomonads and examined at day 15 after infection). Doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximately 10-20 % compared to controls. Gross histopathologic changes at abdominal organs and mortality rate, as a consequence of pathogenicity of the protozoa and the immune response of the host, were evaluated. IFN-gamma and IL-4 cytokines were determined on days -5, 0, 5, 10, and 15 postinfection by indirect ELISA. Treatment with PAL before infection modulates and downregulates the IFN-gamma concentration, while anticipates and upregulates the IL-4 level. The assays performed have showed the utility of the murine model of experimental trichomoniasis for the evaluation of immunomodulatory activity of synthetic or natural products.
引用
收藏
页码:73 / 78
页数:6
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