Enrichment and characterization of dendritic cells from rat renal mesangium

被引:13
作者
Gieseler, R
Hoffmann, PR
Kuhn, R
Fayyazi, A
Stojanovic, T
Schlemminger, R
Peters, JH
机构
[1] Univ Gottingen, Dept Immunol, D-37075 Gottingen, Germany
[2] Univ Gottingen, Dept Pathol, Div Gen Pathol 1, D-37075 Gottingen, Germany
[3] Univ Gottingen, Policlin Gen Surg, D-37075 Gottingen, Germany
关键词
D O I
10.1046/j.1365-3083.1997.d01-175.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dendritic cells (DC) initiate primary immune reactions and are distributed throughout most tissues. The most potent DC population of the kidney has long been suggested to reside within the glomerular mesangium. Using LEW.1A rats, we enriched and characterized such low-density cells. Mesangial DC generally exhibited round to oval cell bodies and cytoplasmic veils. Phenotypically, these cells were 100% OX-6(++), 45% OX-42(++), 35% ED1(low), 10% OX-62(low), and negative for ED2 and alpha-naphtylbutyrate esterase. Introducing a new monoclonal antibody, R3, which stains a subset of splenic DC, we showed strong antigen expression on 60% of mesangial DC. Correlating cell populations were detected immunohistochemically. Functionally, mesangial DC potently stimulated allogeneic mixed leucocyte reactions, but did not phagocytose opsonized Escherichia coli. In addition to their striking phenotypic similarity with autologous splenic DC, mesangial DC exhibited 88% of the allostimulatory activity of splenic DC. Calculation indicated approximately two mesangial DC per glomerulum. We suggest that these cells comprise different maturation-dependent subsets. The OX-62 integrin especially appears to be expressed only on mature mesangial DC, which may correlate to lymphoid veiled cells or interdigitating DC. An employment of mesangial DC in experimental models of acute allograft rejection or glomerulonephritis is discussed.
引用
收藏
页码:587 / 596
页数:10
相关论文
共 37 条
  • [1] MONOCYTE MACROPHAGE DERIVED CELLS IN NORMAL AND TRANSPLANTED HUMAN KIDNEYS
    ALPERS, CE
    BECKSTEAD, JH
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1985, 36 (02): : 129 - 140
  • [2] AUSTYN JM, 1994, J IMMUNOL, V152, P2401
  • [3] AUSTYN JM, 1993, ADV EXP MED BIOL, V329, P489
  • [4] AUSTYN JM, 1988, TRANSPLANT REV, V2, P139
  • [5] EVIDENCE THAT RAT RENAL-ALLOGRAFTS ARE REJECTED BY CYTO-TOXIC T-CELLS AND NOT BY NONSPECIFIC EFFECTORS
    BRADLEY, JA
    MASON, DW
    MORRIS, PJ
    [J]. TRANSPLANTATION, 1985, 39 (02) : 169 - 175
  • [6] THE MRC OX-62 ANTIGEN - A USEFUL MARKER IN THE PURIFICATION OF RAT VEILED CELLS WITH THE BIOCHEMICAL-PROPERTIES OF AN INTEGRIN
    BRENAN, M
    PUKLAVEC, M
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (06) : 1457 - 1465
  • [7] In vitro characterization of rat bone marrow-derived dendritic cells and their precursors
    ChenWoan, M
    Delaney, CP
    Fournier, V
    Wakizaka, Y
    Murase, N
    Fung, J
    Starzl, TE
    Demetris, AJ
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 1996, 59 (02) : 196 - 207
  • [8] COOK HT, 1987, AM J PATHOL, V126, P126
  • [9] IMMUNOENZYMATIC LABELING OF MONOCLONAL-ANTIBODIES USING IMMUNE-COMPLEXES OF ALKALINE-PHOSPHATASE AND MONOCLONAL ANTI-ALKALINE PHOSPHATASE (APAAP COMPLEXES)
    CORDELL, JL
    FALINI, B
    ERBER, WN
    GHOSH, AK
    ABDULAZIZ, Z
    MACDONALD, S
    PULFORD, KAF
    STEIN, H
    MASON, DY
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (02) : 219 - 229
  • [10] DAMOISEAUX JGMC, 1994, IMMUNOLOGY, V83, P140