UV-B-responsive microRNAs in Populus tremula

被引：115

作者：

Jia, Xiaoyun ^{[1
,2
,3
]}

Ren, Ligang ^{[1
,2
]}

Chen, Qi-Jun ^{[1
,2
]}

Li, Runzhi ^{[3
]}

Tang, Guiliang ^{[1
,2
]}

机构：

[1] Univ Kentucky, Gene Suppress Lab, Dept Plant & Soil Sci, Lexington, KY 40546 USA

[2] Univ Kentucky, KTRDC, Lexington, KY 40546 USA

[3] Shanxi Agr Univ, Ctr Agr Biotechnol, Taigu 030801, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2009年 / 166卷 / 18期

关键词：

cis-Element; Gene regulation; MicroRNA; Populus tremula; UV-B radiation; ACTING REGULATORY ELEMENTS; SMALL RNAS; DOWN-REGULATION; STRESS; ARABIDOPSIS; EXPRESSION; GENES; IDENTIFICATION; PLANTACYANIN; BIOGENESIS;

D O I：

10.1016/j.jplph.2009.06.011

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

MicroRNAs (miRNAs) play vital roles in down-regulating gene expression at the post-transcriptional level.. A set of 24 UV-B stress-responsive miRNAs (13 up-regulated and 11 down-regulated) was identified in Populus tremula plantlet by expression profiling with our in-house miRNA filter array. Six of the UV-B-responsive miRNA and their corresponding target genes were verified for their expressions by RNA blotting and quantitative reverse transcription PCR (qRT-PCR), respectively. The predicted target genes for these miRNAs encode diverse proteins including transcription factors and phytohormone signal-related proteins. Promoter analysis of the UV-B-responsive miRNAs revealed the presence of many light-relevant cis-elements. However, these cis-elements were not necessarily specific to the promoters of UV-responsive miRNAs, indicating that other machinery may be involved in the regulation of UV-responsive miRNAs. Finally, a model was developed to describe the potential regulatory networks mediated by the UV-B-responsive miRNAs in P tremula. These results provide new insights into the understanding of miRNAs as ubiquitous regulators in plant response to UV-B and other stresses. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：2046 / 2057

页数：12

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