Duck Tembusu virus detection and characterization from mosquitoes in duck farms, Thailand

Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Flavivirus genus and Flaviviridae family. The transmission of DTUMV involves mosquito vectors; however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. This study was conducted to examine DTMUV detection and characterization from mosquitoes in duck farms in central Thailand. Mosquitoes were collected from two duck farms in Sing Buri Province and two duck farms in Ang Thong Province from September 2015 to July 2016 using four CDC-light traps. A total of 30,841 mosquitoes were collected and identified to seven species (Anopheles (An.) barbirostris, An. stephensi, Culex (Cx.) gelidus, Cx. quinquefasciatus, Cx. tritaeniorhynchus, Mansonia (Ma.) annulifera and Ma. uniformis). The most common collected species from each duck farm and each collection time was Cx. tritaeniorhynchus. Mosquitoes were pooled according to species, location, and collection time and then examined for DTMUV by RT-PCR. A total of 273 mosquito pools were examined, with only one pool of Cx. tritaeniorhynchus collected from Sing Buri Province in November 2015 testing positive for DTMUV. Phylogenetic analysis of the polyprotein genes demonstrated that a mosquito-derived Thai DTMUV was grouped into subcluster 2.1 and most closely related to the 2013 Thai DTMUVs. Thus, this study indicated that Cx. tritaeniorhynchus may play a role as a vector in the transmission of DTMUV in Thailand. However, additional studies concerning the vector competence of this mosquito for DTMUV are needed.