Graphene modified titanium alloy promote the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells

被引：29

作者：

Li, Kewen ^{[1
,2
]}

Yan, Jinhong ^{[1
]}

Wang, Chunhui ^{[3
]}

Bi, Long ^{[1
]}

Zhang, Qi ^{[1
]}

Han, Yisheng ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, Xian, Peoples R China

[2] Qinghai Univ, Affiliated Hosp, Dept Orthopaed, Xining, Peoples R China

[3] Urumqi Ethn Cadre Coll, Dept Mil Med Training, Hutubi 831200, Xinjiang, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 489卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Graphene; Titanium alloy; Surface modification; Bioactivity; MESENCHYMAL STEM-CELLS; IMPLANTS; NANOMATERIALS; TOPOGRAPHY; OSTEOBLAST; TI6AL4V; CULTURE;

D O I：

10.1016/j.bbrc.2017.05.124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effects of graphene coating on improving the biological activity of a titanium alloy (Ti6Al4V) widely used in hip and knee joint replacements. The experiments included immunofluorescence staining for observing cellular adhesion, Cell Counting Kit-8 (CCK-8) for evaluating cellular proliferation and reverse transcription-polymerase chain reaction (RT-PCR) for detecting the differentiation of bone marrow stromal cells on different scaffolds. The results showed that G-Ti6Al4V exhibited a higher mean integrated optical density (IOD) for vinculin and resulted in a higher cell proliferation rate and higher osteoblast-specific gene transcription levels. In summary, graphene could be used as a new nanocoating material for Ti6Al4V scaffolds to enhance their surface bioactivity. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：187 / 192

页数：6

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