Development and validation of an automated extraction method (accelerated solvent extraction®) and a reverse-phase HPLC analysis method for assay of ivermectin in a meat-based chewable formulation

被引：21

作者：

Abend, AM

Chung, L

McCollum, DG

Wuelfing, WP

机构：

[1] Merck & Co Inc, Merck Res Labs, W Point, PA 19486 USA

[2] Merck & Co Inc, Mfg Div, W Point, PA 19486 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 31卷 / 06期

关键词：

extraction; accelerated solvent extraction; ivermectin; HEARTGARD CHEWABLES; reversed-phase HPLC;

D O I：

10.1016/S0731-7085(03)00020-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for monitoring ivermectin content in HEARTGARD CHEWABLES(R) has been developed and validated. The method consists of the automated extraction of ivermectin from the meat-based formulation under conditions of elevated temperature and pressure (accelerated solvent extraction, ASE(R)), and determination of the active by reverse-phase high performance liquid chromatography (HPLC). The method resolves both active species of ivermectin (components H2B1a and H2B1b) from the formulation matrix. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：1177 / 1183

页数：7

共 6 条

[1] Determination of felodipine in tablets using accelerated solvent extraction [J].