Cloning of a breakpoint cluster region on chromosome 14 in uterine leiomyoma

被引：8
作者：
Bhugra, B
Smolarek, TA
Lynch, RA
Meloni, AM
Sandberg, AA
Deaven, L
Menon, AG
机构：
[1] Univ Cincinnati, Coll Med, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA
[2] Univ Utah, Salt Lake City, UT 84112 USA
[3] Genzyme Genet, Scottsdale, AZ 85251 USA
[4] Univ Calif Los Alamos Natl Lab, Ctr Human Genome Studies, Los Alamos, NM 87545 USA
来源：
CANCER LETTERS | 1998年 / 126卷 / 02期
关键词：
breakpoint cluster region; chromosome; 14; uterine leiomyoma;
D O I：
10.1016/S0304-3835(97)00478-3
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
A recurrent reciprocal chromosomal translocation, t(12;14)(q15;q24) is frequently observed in uterine leiomyoma. Chromosome 12 breakpoints have been shown to occur in a region of approximately 150 kb that contains the gene for a high mobility group protein (HMGI-C). The breakpoint region on chromosome 14 has not been precisely defined. We have generated a contig of overlapping yeast artificial chromosome (YAC) clones approximately 3 Mb in size. Fluorescence in situ hybridization (FISH) analysis showed that this contig spanned the t(12;14) breakpoints in three uterine leiomyomas and that the breakpoints in these tumors occurred within a 1 Mb region. A 30 kb cosmid spanning one of the breakpoints was isolated to set the stage for identifying regions on chromosome 14 that may cause this region to be a preferential site for chromosomal translocation. Published by Elsevier Science Ireland Ltd.
引用
收藏
页码：119 / 126
页数：8
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