Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of chilli veinal mottle virus

被引：19

作者：

Banerjee, Amrita ^{[1
]}

Roy, Somnath ^{[2
]}

Sharma, Susheel Kumar ^{[3
]}

Dutta, Sudip Kumar ^{[4
]}

Chandra, Satish ^{[1
]}

Ngachan, S. V. ^{[1
]}

机构：

[1] ICAR Res Complex North Eastern Hill Reg, Umiam 793103, Meghalaya, India

[2] ICAR Natl Bur Plant Genet Resources, Reg Stn, Umiam 793103, Meghalaya, India

[3] ICAR Res Complex NEH Reg, Manipur Ctr, Lamphelpat 795004, Manipur, India

[4] ICAR Res Complex NEH Reg, Mizoram Ctr, Kolasib 796081, Mizoram, India

来源：

ARCHIVES OF VIROLOGY | 2016年 / 161卷 / 07期

关键词：

VEIN BANDING VIRUS; MOSAIC-VIRUS; 1ST REPORT; PEPPER; INDIA;

D O I：

10.1007/s00705-016-2850-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chilli veinal mottle virus (ChiVMV) causes significant economic loss to chilli cultivation in northeastern India, as well as in eastern Asia. In this study, we have developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid, sensitive and specific diagnosis of ChiVMV. Amplification could be visualized after adding SYBR Green I (10009) dye within 60 min under isothermal conditions at 63 degrees C, with a set of four primers designed based on the large nuclear inclusion protein (NIb) domain of ChiVMV (isolate KC-ML1). The RT-LAMP method was 100 times more sensitive than one-step reverse transcription polymerase chain reaction (RT-PCR), with a detection limit of 0.0001 ng of total RNA per reaction.

引用

页码：1957 / 1961

页数：5

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