Role of Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mice in vitro

被引：4
作者：
Bahena, I. [1 ]
Xu, E. [2 ]
Betancourt, M. [1 ]
Casas, E. [1 ]
Ducolomb, Y. [1 ]
Gonzalez, C. [1 ]
Bonilla, E. [1 ,2 ]
机构：
[1] Univ Autonoma Metropolitana Iztapalapa, Dept Ciencias Salud, Mexico City 09340, DF, Mexico
[2] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA
来源：
ZYGOTE | 2014年 / 22卷 / 04期
关键词：
ESC; Germ cells; Mael; Oogenesis; RNAi; siRNA; TRANSPOSON CONTROL; MOUSE MAELSTROM; GENE-EXPRESSION; OOCYTES; DERIVATION; SPERMATOGENESIS; PLURIPOTENCY; MAINTENANCE; FOLLICLES; PATHWAY;
D O I：
10.1017/S0967199412000743
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
In a previous study, we have identified a set of conserved spermatogenic genes whose expression is restricted to testis and ovary and that are developmentally regulated. One of these genes, the transcription factor Mael, has been reported to play an essential role in mouse spermatogenesis. Nevertheless, the role of Mael in mouse oogenesis has not been defined. In order to analyse the role of Mael in mouse oogenesis, the expression of this gene was blocked during early oogenesis in mouse in vitro using RNAi technology. In addition, the role of Mael during differentiation of embryonic stem cells (ESC) into germ cells in vitro was analysed. Results show that downregulation of Mael by a specific short interfering RNA disrupted fetal oocyte growth and differentiation in fetal ovary explants in culture and the expression of several germ-cell markers in ESC during their differentiation. These results suggest that there is an important role for Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mouse in vitro.
引用
收藏
页码：513 / 520
页数：8
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