Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil

被引：2

作者：

Magalhaes-Matos, Paulo Cesar ^{[1
]}

Cunha-Santos, Rafaelle ^{[1
]}

Silva Sousa, Paulo Geovani ^{[1
]}

Sampaio-Junior, Francisco Dantas ^{[1
]}

Leite Barros, Flavia de Nazare ^{[1
]}

Paixao Mourao, Fabio Rodrigo ^{[2
]}

Lima Oliveira, Wanessa Batista

Gabriel, Aurea Martins ^{[3
]}

Monteiro, Silvia Gonzalez ^{[4
]}

Goes-Cavalcante, Gustavo ^{[1
]}

Scofield, Alessandra ^{[1
]}

机构：

[1] Univ Fed Para UFPA, Inst Med Vet, Lab Parasitol Anim, Campus Castanhal,BR 316,Km 61, BR-68740080 Castanhal, PA, Brazil

[2] Secretaria Municipal Saude Municipio Macapa, Coordenadoria Vigilancia Ambiental, Dept Zoonoses, Macapa, AM, Brazil

[3] Univ Fed Para UFPA, Inst Tecnol, Fac Engn Sanit & Ambiental, Belem, Para, Brazil

[4] Univ Fed Santa Maria, CCS, Dept Microbiol & Parasitol MIP, BR-97119900 Santa Maria, RS, Brazil

来源：

CIENCIA RURAL | 2016年 / 46卷 / 04期

关键词：

Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon; COATI NASUA-NASUA; PANTANAL REGION; INFECTION; CRUZI;

D O I：

10.1590/0103-8478cr20150679

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapa and Para, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapa and Para. Whole blood samples were collected and kept at -20 degrees C for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crabeating raccoons and captive and free-living coatis in Para State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Para State, being this the first such report in P. cancrivorus.

引用

页码：663 / 668

页数：6

共 20 条

[1]

Abdel-Rady A, 2008, VET WORLD, V1, P325

[2]

Boehringer E., 1961, An. Inst. Med. Regional, V5, P69

[3]

Claes Filip, 2004, Kinetoplastid Biol Dis, V3, P3

[4]

Colpo Cristina Braccini, 2005, Cienc. Rural, V35, P717, DOI 10.1590/S0103-84782005000300038

[5]

DEANE LEONIDAS M., 1961, REV INST MED TROP SAO PAULO, V3, P15

[6]

EISENBERG J.F., 1999, MAMMALS NEOTROPICS C, P287

[7]

Emmons L.H., 1997, NEOTROPICAL RAINFORE

[8] The coati (Nasua nasua, Carnivora, Procyonidae) as a reservoir host for the main lineages of Trypanosoma cruzi in the Pantanal region, Brazil [J].

Herrera, H. M. ;

Lisboa, C. V. ;

Pinho, A. P. ;

Olifiers, N. ;

Bianchi, R. C. ;

Rocha, F. L. ;

Mourao, G. M. ;

Jansen, A. M. .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2008, 102 (11) :1133-1139

[9] Food web connections and the transmission cycles of Trypanosoma cruzi and Trypanosoma evansi (Kinetoplastida, Trypanosomatidae) in the Pantanal Region, Brazil [J].

Herrera, H. M. ;

Rocha, F. L. ;

Lisboa, C. V. ;

Rademaker, V. ;

Mourao, G. M. ;

Jansen, A. M. .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2011, 105 (07) :380-387

[10] Enzootiology of Trypanosoma evansi in Pantanal, Brazil [J].

Herrera, HM ;

Dávila, AMR ;

Norek, A ;

Abreu, UG ;

Souza, SS ;

D'Andrea, PS ;

Jansen, AM .

VETERINARY PARASITOLOGY, 2004, 125 (3-4) :263-275

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