Phenolic, flavonoid contents, anticholinesterase and antioxidant evaluation of Iris germanica var; florentina

被引：66

作者：

Ullah, Farhat ^{[1
]}

Ayaz, Muhammad ^{[1
]}

Sadiq, Abdul ^{[1
]}

Hussain, Abid ^{[1
]}

Ahmad, Sajjad ^{[1
]}

Imran, Muhammad ^{[1
]}

Zeb, Anwar ^{[1
]}

机构：

[1] Univ Malakand, Dept Pharm, Dir Lower 18000, Khyber Pakhtoon, Pakistan

来源：

NATURAL PRODUCT RESEARCH | 2016年 / 30卷 / 12期

关键词：

Acetylcholinesterase (AChE); butyrylcholinesterase (BChE); DPPH; H2O2; ABTS; Ellman's assay; Iris germanica; IN-VITRO; AFFINITY RELATIONSHIP; ISOFLAVONOIDS; DYSFUNCTION; INHIBITION; FRACTIONS; EXTRACTS; SAPONINS;

D O I：

10.1080/14786419.2015.1057585

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was designed to investigate antioxidant and anticholinesterase potential of Iris germanica var; florentina. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory potential of plant samples were investigated by Ellman's assay. Antioxidant activity was performed using DPPH, H2O2 and ABTS free radical scavenging assays. Total phenolics and flavonoids contents were expressed in mg GAE/g dry weight and mg RTE/g, respectively. In AChE inhibition assay, Ig.Fl, Ig.Sp and Ig.Cf fractions exhibited highest activity with IC50 values of <0.1, 5.64 and 19 mu g/mL, respectively. In BChE inhibitory assay, Ig.Fl, Ig.Sp, Ig.Cf and Ig.Cr were most active with IC50 of < 0.1, < 0.1, 31 and 78 mu g/mL, respectively. In DPPH assay, Ig.Fl and Ig.Cf exhibited highest inhibition of free radicals, 80.52% (IC50 = 9 mu g/mL) and 78.30% (IC50 = 8 mu g/mL), respectively. In ABTS assay Ig.Cr, Ig.Cf, Ig.Fl and Ig.Sp exhibited IC50 values of < 0.1, 2, 2 and 3 mu g/mL, respectively.

引用

页码：1440 / 1444

页数：5

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