First Report of Botryosphaeria dothidea causing leaf necrosis of Camellia sinensis in Fujian Province, China

被引:13
|
作者
Jayawardena, R. S. [1 ,2 ]
Li, X. H. [1 ]
Xu, W. [1 ]
Yan, J. Y. [1 ]
Li, H. L. [3 ]
Hyde, K. D. [2 ]
机构
[1] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
[2] Mae Fah Luang Univ, Ctr Excellence Fungal Res, Sch Sci, Chiang Rai 57100, Thailand
[3] Fujian Acad Agr Sci, Tea Res Inst, Fuan 355015, Peoples R China
关键词
D O I
10.1094/PDIS-08-15-0930-PDN
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Camellia sinensis (L.) Kuntze (Theaceae), commonly known as tea, is a major economic crop cultivated in China (Maharachchikumbura et al. 2013). A cultivar of C. sinensis, purple rose, is commonly grown in the tea base of Zhangzhou, Fujian Province. In June 2015, a leaf necrosis was observed on this cultivar in different farms of different counties in Fujian Province. Symptoms began as small brown lesions on young and mature leaves, becoming necrotic, 2 to 4 cm diameter with a brown margin. To determine the causal agent, symptomatic leaf tissue was collected from plants from Zhangzhou in June 2015. Small pieces from the margin of the necrosis were surface sterilized in 75% alcohol for 2 min, 5% NaClO for 1 min, rinsed twice in distilled water, plated onto potato dextrose agar (PDA), and incubated at 25°C for 5 days. Among the isolated pathogens (such as Colletotrichum) from the leaves, several strains (8) were identified as Botryosphaeria dothidea (Moug.: Fr.) Ces. & De Not. from morphological characters. The cultures produced white-gray fluffy mycelia, black from below, becoming dark brown with age. Conidiomata were produced after 10 days on PDA. Conidiogenous cells were hyaline, subcylindrical, and 4 to 16 × 2 to 5 µm (n = 20). Conidia were 14.6 to 26.1 × 4.8 to 8.8 µm (n = 40), hyaline, unicellular, narrowly fusiform, with a subtruncate to bluntly rounded base, forming a septum before germination, and smooth walled with a granular content. DNA was amplified using rDNA-ITS primer pair ITS1/ITS4 (White et al. 1990) and tef1 728F/986R (Carbone and Kohn 1999; Hyde et al. 2014). Maximum parsimony analysis (MP) using PAUP (phylogenetic analysis using parsimony) v. 4.0b10 and a Bayesian inference (BI) was used to construct the phylogenies using Mr. Bayes v. 3.1.2 to confirm the identity of the isolates. Two representative isolates JZB310001 and JZB310002 clustered together with B. dothidea (CBS 115476) with 90% bootstrap support and 0.98 Bayesian posterior probabilities (PP) support. Sequences of the two isolates have been deposited in GenBank under KT459354, KT459355, KT459356, and KT459357, respectively. Based on the above morphological and molecular data, the two isolates were identified as B. dothidea. In order to confirm the pathogenicity, Koch’s postulates were conducted using two methods. In the first method, 5 ml of a conidial suspension (1 × 106 conidia/ml) was sprayed onto 10 detached leaves; five with wounds and five unwounded. Control leaves (five) were sprayed with distilled water. The second method used mycelium plugs (Yan et al. 2013) of actively growing colony of B. dothidea on 10 detached twigs, five with wound and five unwounded. The control twigs (five) were treated with sterile PDA mycelium plug. The inoculated leaves and twigs were maintained in a greenhouse at 28°C at a constant relative humidity of 95%. Brown necrotic lesions appeared on leaves and twigs after 7 days, while the control plants remained healthy. The experiments were conducted three times and the fungus was reisolated was identified as B. dothidea by morphology and molecular data. This is the first report of B. dothidea causing tea leaf necrosis in China. Tea growers should pay more attention in the incidence of this disease. © 2016, American Phytopathological Society. All rights reserved.
引用
收藏
页码:854 / 854
页数:1
相关论文
共 50 条
  • [31] First Report of Colletotriclzum fructicola Causing Anthracnose on Camellia sinensis in Guangdong Province, China
    Shi, N. N.
    Du, Y. X.
    Ruan, H. C.
    Yang, X. J.
    Dai, Y. L.
    Gan, L.
    Chen, F. R.
    PLANT DISEASE, 2018, 102 (01) : 241 - 242
  • [32] First Report of Alternaria alternata Causing Leaf Spots of Tea (Camellia sinensis) in China
    Zhou, L. X.
    Xu, W. X.
    PLANT DISEASE, 2014, 98 (05) : 697 - 697
  • [33] First Report of Pseudomonas cichorii Causing Tomato Pith Necrosis in Fujian Province, China
    Ruan, H.
    Shi, N.
    Du, Y.
    Chen, F.
    Yang, X.
    Gan, L.
    Dai, Y.
    PLANT DISEASE, 2019, 103 (01) : 145 - 145
  • [34] First Report of Leaf Blight on Mahonia fortunei Caused by Botryosphaeria dothidea in China
    Huang, Z. D.
    Wang, J. Y.
    Yin, C. P.
    Gui, L.
    Zhang, Y. L.
    PLANT DISEASE, 2023, 107 (02) : 558 - 558
  • [35] First Report of Leaf Blight of Syringa oblata Caused by Botryosphaeria dothidea in China
    Zhi, Xiaotan
    Rong, Xinyue
    Liang, Hongchun
    Shan, Hongyin
    Chen, Zhaorong
    PLANT DISEASE, 2024, 108 (11)
  • [36] First Report of Leaf Blotch of Salix babylonica Caused by Botryosphaeria dothidea in China
    Ju, Yue
    Si, Yuan-Zhi
    Li, De-Wei
    Xu, Wu
    Sun, Jian-Wei
    Zhu, Li-Hua
    PLANT DISEASE, 2021, 105 (01) : 224 - 224
  • [37] First Report of Shoot and Leaf Blight Caused by Botryosphaeria dothidea on Taxus X media in Sichuan Province, China
    Wang, Ying
    Chen, Jie
    Zhang, Boyang
    Xie, Xiulan
    Ren, Maozhi
    PLANT DISEASE, 2023, 107 (09) : 2852 - 2852
  • [38] First Report of Leaf Spot of Cornus alba Caused by Botryosphaeria dothidea in China
    Cai, Y. N.
    Wang, K. Q.
    Zhang, X.
    Li, Y. G.
    PLANT DISEASE, 2020, 104 (01) : 282 - 282
  • [39] First Report of Botryosphaeria dothidea Causing Fruit Rot of Rellowhorn (Xanthoceras sorbifolium) in China
    Liu, X.
    Liu, H. X.
    Han, X. L.
    Zhang, Y. L.
    Liang, Q.
    Li, S. K.
    Yang, K. Q.
    PLANT DISEASE, 2018, 102 (08) : 1662 - 1662
  • [40] First Report of Botryosphaeria dothidea Causing Canker and Shoot Blight of Eucalyptus in China.
    Yu, L.
    Chen, X. L.
    Gao, L. L.
    Chen, H. R.
    Huang, Q.
    PLANT DISEASE, 2009, 93 (07) : 764 - 764