The transcriptional factor Osterix directly interacts with RNA helicase A

被引：15
作者：
Amorim, Bruna Rabelo
Okamura, Hirohiko [1 ]
Yoshida, Kaya
Qiu, Lihong
Morimoto, Hiroyuki
Haneji, Tatsuji
机构：
[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Histol & Oral Histol, Tokushima 7708504, Japan
[2] China Med Univ, Sch Stomatol, Dept Endodont, Shenyang 11002, Peoples R China
[3] Univ Occupat & Environm Hlth, Sch Med, Dept Anat 2, Kitakyushu, Fukuoka 8078555, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 355卷 / 02期
关键词：
Osterix; RNA helicase A; MALDI-TOF;
D O I：
10.1016/j.bbrc.2007.01.150
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：347 / 351
页数：5
相关论文
共 24 条
[1]   RNA, helicases: Regulators of differentiation [J].
Abdelhaleem, M .
CLINICAL BIOCHEMISTRY, 2005, 38 (06) :499-503
[2]   Dual roles of RNA helicase A in CREB-dependent transcription [J].
Aratani, S ;
Fujii, R ;
Oishi, T ;
Fujita, H ;
Amano, T ;
Ohshima, T ;
Hagiwara, M ;
Fukamizu, A ;
Nakajima, T .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (14) :4460-4469
[3]   Sp1 and kruppel-like factor family of transcription factors in cell growth regulation and cancer [J].
Black, AR ;
Black, JD ;
Azizkhan-Clifford, J .
JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 188 (02) :143-160
[4]   Regulation of the activity of Sp1-related transcription factors [J].
Bouwman, P ;
Philipsen, S .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2002, 195 (1-2) :27-38
[5]   The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation [J].
Caretti, Giuseppina ;
Schiltz, R. Louis ;
Dilworth, F. Jeffrey ;
Di Padova, Monica ;
Zhao, Po ;
Ogryzko, Vasily ;
Fuller-Pace, Frances V. ;
Hoffman, Eric P. ;
Tapscott, Stephen J. ;
Sartorelli, Vittorio .
DEVELOPMENTAL CELL, 2006, 11 (04) :547-560
[6]   Osx transcriptional regulation is mediated by additional pathways to BMP2/Smad signaling [J].
Celil, AB ;
Hollinger, JO ;
Campbell, PG .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2005, 95 (03) :518-528
[7]   DExD/H box RNA helicases: multifunctional proteins with important roles in transcriptional regulation [J].
Fuller-Pace, Frances V. .
NUCLEIC ACIDS RESEARCH, 2006, 34 (15) :4206-4215
[8]   Molecular cloning, structure, expression, and chromosomal localization of the human Osterix (SP7) gene [J].
Gao, YG ;
Jheon, A ;
Nourkeyhani, H ;
Kobayashi, H ;
Ganss, B .
GENE, 2004, 341 :101-110
[9]   Unravelling the complexities of the NF-κB signalling pathway using mouse knockout and transgenic models [J].
Gerondakis, S. ;
Grumont, R. ;
Gugasyan, R. ;
Wong, L. ;
Isomura, I. ;
Ho, W. ;
Banerjee, A. .
ONCOGENE, 2006, 25 (51) :6781-6799
[10]  
Hatta M, 2006, INT J MOL MED, V17, P425
123