Three-dimensional organization of microtubules in tumor cells studied by confocal laser scanning microscopy and computer-assisted deconvolution and image reconstruction

被引:14
作者
Strohmaier, AR
Porwol, T
Acker, H
Spiess, E
机构
[1] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany
[2] Deutsch Krebsforschungszentrum, D-6900 Heidelberg, Germany
关键词
reconstruction; three-dimensional; confocal microscopy; deconvolution; microtubule;
D O I
10.1159/000016760
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Confocal microscopy, image deconvolution and computer-assisted methods have been used to reconstruct the three-dimensional (3-D) distribution of tubulin in cells. The techniques were applied to tumor cells growing under regular culture conditions (planar cultivation) and those penetrating into reconstituted collagen matrices (spatial cultivation). As expected the application of deconvolution algorithms enhanced the resolution of results. The deconvolution using the maximum likelihood estimation included the measurement of the point spread function of the optical setup. The data visualization of the resulting data sets uses volume as well as surface rendering approaches. The 3-D reconstruction gives a clear image of the spatial arrangement of tubulin fibers in relation to cell shape and position of other cellular organelles, particularly the nucleus. The tubulin forms an intricate network of fibers of variable thickness. The highest tubulin concentrations appear in the cell periphery and particularly in pseudopodia/invadopodia. This is indicative of an enhanced transport of intracellular material facilitating cell movement and lysis of the extracellular matrix. The investigation is assumed to stimulate further experiments using a variety of techniques leading to the complete understanding of the spatial architecture of living cells. Copyright (C) 2000 S. Karger AG, Basel.
引用
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页码:1 / 8
页数:8
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