This study was carried out to investigate the effects of different types of beta-cycloclextrin (beta-CD) treatments on chemical and sensory characteristics of cholesterol-reduced cream cheese. The cholesterol removal rates were 92.0% in cream cheese treated by powder beta-CD, and 82.6% in cream cheese treated by crosslinked beta-M. Amounts of short-chain fatty acid and free amino acids were significantly lower in cream cheese made by crosslinked beta-CD-treated milk, especially after 2 weeks storage, compared with those of no beta-CD-treated control and cream cheese made by powder beta-CD treated milk. Among rheological properties, cohesiveness was significantly higher, and gumminess in cream cheese made by crosslinked beta-CID-treated milk was slightly lower than others. In sensory analysis, no difference was found in texture among treatments, while bitterness was lower in the early stage of storage, and overall quality was higher score, in cream cheese made by crosslinked beta-CD-treated cream at 3 and 4 week storage, compared with those in control and powder beta-CD-treated group. The present study indicated that crosslinked beta-CD treatment resulted in an efficient cholesterol removal rate over 80% and a deceleration of ripening, which may provide a longer shelf life without significant adverse effects in chemical and sensory properties.