Development of EST-SSRs by an efficient FIASCO-based strategy:: A case study in rare minnow (Gobiocyrpis rarus)

被引：6

作者：

Cheng, L.

Liao, X.

Yu, X.

Tong, J. ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China

来源：

ANIMAL BIOTECHNOLOGY | 2007年 / 18卷 / 03期

基金：

中国国家自然科学基金;

关键词：

cDNA library; expressed sequence tag; FIASCO; fish; microsatellite;

D O I：

10.1080/10495390601054980

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs) contained targeted CA-repeats, in which 29 unique EST-SSRs were identified. Ten out of the 28 loci for which primer pairs were designed were polymorphic with alleles ranging from three to seven (mean 4.50). Some of these EST-SSRs can be amplified in other species. These results proved that cDNA-FIASCO is an efficient way to isolate novel EST-SSRs in a fish.

引用

页码：143 / 152

页数：10

共 25 条

[1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].