Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake

被引：103

作者：

Ding, Xiang ^{[1
]}

Tang, Jie ^{[1
]}

Cao, Mei ^{[2
]}

Guo, Chun-xiao ^{[1
]}

Zhang, Xia ^{[1
]}

Zhong, Jing ^{[1
]}

Zhang, Jie ^{[1
]}

Sun, Qun ^{[1
]}

Feng, Su ^{[1
]}

Yang, Zhi-rong ^{[1
]}

Zhao, Jian ^{[1
,2
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Biol Resource & Ecol Environm, Chengdu 610064, Peoples R China

[2] Sichuan Prov Peoples Hosp, Sichuan Acad Med Sci, Key Lab, Chengdu 610072, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 47卷 / 02期

关键词：

Polysaccharide structure; Antioxidant assay; Tricholoma matsutake; PURIFICATION; GLUCANS;

D O I：

10.1016/j.ijbiomac.2010.04.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4-beta-D-glucopyranose residue which branches at 0-6 based on the experimental results. The branches were mainly composed of an (1 -> 3)-alpha-D-galactopyranose residue, and terminated with alpha-D-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH- radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：271 / 275

页数：5

共 24 条

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