Multi-step evolution of protein conformation on electrospray into the gas phase

被引：11

作者：

McLafferty, Fred W. ^{[1
]}

Castro, Sergio ^{[1
]}

Breuker, Kathrin ^{[2
,3
]}

机构：

[1] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

[2] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

[3] Univ Innsbruck, Ctr Mol Biosci Innsbruck, A-6020 Innsbruck, Austria

来源：

EUROPEAN JOURNAL OF MASS SPECTROMETRY | 2010年 / 16卷 / 03期

关键词：

gaseous protein conformation; H/D exchange; etectrospray; protein folding; NATIVE CYTOCHROME-C; CAPTURE DISSOCIATION; MASS-SPECTROMETRY; STRUCTURAL-CHARACTERIZATION; TOP-DOWN; IONS; IONIZATION; TRANSITION; COMPLEXES; MOLECULE;

D O I：

10.1255/ejms.1058

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

In the gas phase, some properties of native versus denatured protein conformations correspond to those in solution, such as affinity for protons and physical cross section. However, the capacity for hydrogen/deuterium exchange is the opposite, with ubiquitin 7+ and 13+ ions exchanging >60D and -15D atoms, respectively. A variety of experimental methods now delineate a series of conformational perturbations that can occur in the 10(-12)s to 10(+2)s following electrospray, including side-chain collapse, hydrophobic and electrostatic non-covalent bond unfolding and refolding into a variety of non-native structures.

引用

页码：437 / 442

页数：6

共 32 条

[1] Evidence for unfolding and refolding of gas-phase cytochrome c ions in a Paul trap [J].

Badman, ER ;

Myung, S ;

Clemmer, DE .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2005, 16 (09) :1493-1497

[2] The thermal unfolding of native cytochrome c in the transition from solution to gas phase probed by native electron capture dissociation [J].

Breuker, K ;

McLafferty, FW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (31) :4911-4914

[3] Detailed unfolding and folding of gaseous ubiquitin ions characterized by electron capture dissociation [J].

Breuker, K ;

Oh, HB ;

Horn, DM ;

Cerda, BA ;

McLafferty, FW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) :6407-6420

[4] Native electron capture dissociation for the structural characterization of noncovalent interactions in native cytochrome c [J].

Breuker, K ;

McLafferty, FW .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (40) :4900-4904

[5] Stepwise evolution of protein native structure with electrospray into the gas phase, 10-12 to 102 S [J].

Breuker, Kathrin ;

McLafferty, Fred W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18145-18152

[6] PROBING CONFORMATIONAL-CHANGES IN PROTEINS BY MASS-SPECTROMETRY [J].

CHOWDHURY, SK ;

KATTA, V ;

CHAIT, BT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (24) :9012-9013

[7] Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories [J].

Chung, Hoi Sung ;

Louis, John M. ;

Eaton, William A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (29) :11837-11844

[8] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[9] Gas-phase bovine ubiquitin cation conformations resolved by gas-phase hydrogen/deuterium exchange rate and extent [J].

Freitas, MA ;

Hendrickson, CL ;

Emmett, MR ;

Marshall, AG .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 1999, 185 :565-575

[10] Internal energy and fragmentation of ions produced in electrospray sources [J].

Gabelica, V ;

De Pauw, E .

MASS SPECTROMETRY REVIEWS, 2005, 24 (04) :566-587

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