Synthesis of a biotin-tagged photoaffinity probe of 2-azetidinone cholesterol absorption inhibitors

被引：26

作者：

Frick, W ^{[1
]}

Bauer-Schäfer, A ^{[1
]}

Bauer, J ^{[1
]}

Girbig, F ^{[1
]}

Corsiero, D ^{[1
]}

Heuer, H ^{[1
]}

Kramer, W ^{[1
]}

机构：

[1] Aventis Pharma Deutschland GmbH, Dis Grp Metab Dis Ind Pk Hochst, D-65926 Frankfurt, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2003年 / 11卷 / 08期

关键词：

D O I：

10.1016/S0968-0896(03)00047-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The design and synthesis of a biotin-tagged photoreactive analogue C-4 of the cholesterol absorption inhibitor Ezetimibe is described. Photoaffinity labeling of intestinal brush border membrane vesicles with C-4 and subsequent streptavidin-biotin chromatography leads to selective extraction of a 145 kDa integral membrane protein as the molecular target for cholesterol absorption inhibitors. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：1639 / 1642

页数：4

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