Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy

被引：13

作者：

de Rocquigny, Hugues ^{[1
]}

El Meshri, Salah Edin ^{[1
]}

Richert, Ludovic ^{[1
]}

Didier, Pascal ^{[1
]}

Darlix, Jean-Luc ^{[1
]}

Mely, Yves ^{[1
]}

机构：

[1] Fac Pharm, Lab Biophoton & Pharmacol, UMR CNRS 7213, F-67401 Illkirch Graffenstaden, France

来源：

VIRUS RESEARCH | 2014年 / 193卷

关键词：

HIV; NC; Microscopy; Fluorescence; Assembly; FRET-FLIM; Gag; IMMUNODEFICIENCY-VIRUS TYPE-1; RESONANCE ENERGY-TRANSFER; LIFETIME IMAGING MICROSCOPY; MURINE LEUKEMIA-VIRUS; MAJOR HOMOLOGY REGION; ROUS-SARCOMA-VIRUS; PLASMA-MEMBRANE; ZINC-FINGER; GENOMIC RNA; CORRELATION SPECTROSCOPY;

D O I：

10.1016/j.virusres.2014.06.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Gag precursor of HIV-1, formed of the four proteic regions matrix (MA), capsid (CA), nucleocapsid (NC) and p6, orchestrates virus morphogenesis. This complex process relies on three major interactions, NC-RNA acting as a scaffold, CA-CA and MA-membrane that targets assembly to the plasma membrane (PM). The characterization of the molecular mechanism of retroviral assembly has extensively benefited from biochemical studies and more recently an important step forward was achieved with the use of fluorescence-based techniques and fluorescently labeled viral proteins. In this review, we summarize the findings obtained with such techniques, notably quantitative-based approaches, which highlight the role of the NC region in Gag assembly. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：78 / 88

页数：11

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