A smart fluorescence nanoprobe for the detection of cellular alkaline phosphatase activity and early osteogenic differentiation

被引：35
作者：
Cao, Feng-Yi
Fan, Jin-Xuan
Long, Yue
Zeng, Xuan
Zhang, Xian-Zheng [1 ,2 ]
机构：
[1] Wuhan Univ, Minist Educ, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China
[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
来源：
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2016年 / 12卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Fluorescence nanoprobe; Stem cell; Alkaline phosphatase; Osteogenic differentiation; MESENCHYMAL STEM-CELLS; MAGNETIC-RESONANCE; CULTURE; KINASE; SHELL; CORE;
D O I：
10.1016/j.nano.2016.01.010
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
In the past decades, biomaterials were designed to induce stem cell toward osteogenic differentiation. However, conventional methods for evaluation osteogenic differentiation all required a process of cell fixation or lysis, which induce waste of a large number of cells. In this study, a fluorescence nanoprobe was synthesized by combining phosphorylated fluoresceinamine isomer I (FLA) on the surface of mesoporous silica-coated superparamagnetic iron oxide (Fe3O4@mSiO(2)) nanoparticles. In the presence of alkaline phosphatase (ALP), the phosphorylated FLA on the nanoprobe would be hydrolyzed, resulting in a fluorescence recovery of FLA. During early osteogenic differentiation, a high-level expression of cellular ALP was induced, which accelerated the hydrolysis of phosphorylated FLA, resulting in an enhancement of cellular fluorescence intensity. This fluorescence nanoprobe provides us a rapid and non-toxic method for the detection of cellular ALP activity and early osteogenic differentiation. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：1313 / 1322
页数：10
相关论文
共 32 条
[1]  
Anker PSI, 2003, HAEMATOLOGICA, V88, P845
[2]   Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation [J].
Asakura, A ;
Komaki, M ;
Rudnicki, MA .
DIFFERENTIATION, 2001, 68 (4-5) :245-253
[3]   Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term In vitro culture and do not exhibit telomere maintenance mechanisms [J].
Bernardo, Maria Ester ;
Zaffaroni, Nadia ;
Novara, Francesca ;
Cometa, Angela Maria ;
Avanzini, Maria Antonietta ;
Moretta, Antonia ;
Montagna, Daniela ;
Maccario, Rita ;
Villa, Raffaella ;
Daidone, Maria Grazia ;
Zuffardi, Orsetta ;
Locatelli, Franco .
CANCER RESEARCH, 2007, 67 (19) :9142-9149
[4]   Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow [J].
Campagnoli, C ;
Roberts, IAG ;
Kumar, S ;
Bennett, PR ;
Bellantuono, I ;
Fisk, NM .
BLOOD, 2001, 98 (08) :2396-2402
[5]   Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing [J].
Chamberlain, Giselle ;
Fox, James ;
Ashton, Brian ;
Middleton, Jim .
STEM CELLS, 2007, 25 (11) :2739-2749
[6]   A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells [J].
Choi, Chun Kit K. ;
Li, Jinming ;
Wei, Kongchang ;
Xu, Yang J. ;
Ho, Lok Wai C. ;
Zhu, Meiling ;
To, Kenneth K. W. ;
Choi, Chung Hang J. ;
Bian, Liming .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (23) :7337-7346
[7]   STRUCTURE AND MECHANISM OF ALKALINE-PHOSPHATASE [J].
COLEMAN, JE .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1992, 21 :441-483
[8]   Influence of Nanohelical Shape and Periodicity on Stem Cell Fate [J].
Das, Rajat K. ;
Zouani, Omar F. ;
Labrugere, Christine ;
Oda, Reiko ;
Durrieu, Marie-Christine .
ACS NANO, 2013, 7 (04) :3351-3361
[9]   High-Resolution Cellular MRI: Gadolinium and Iron Oxide Nanoparticles for in-Depth Dual-Cell Imaging of Engineered Tissue Constructs [J].
Di Corato, Riccardo ;
Gazeau, Florence ;
Le Visage, Catherine ;
Fayol, Delphine ;
Levitz, Pierre ;
Lux, Francois ;
Letourneur, Didier ;
Luciani, Nathalie ;
Tillement, Olivier ;
Wilhelm, Claire .
ACS NANO, 2013, 7 (09) :7500-7512
[10]   Ultrasmall superparamagnetic iron oxide nanoparticle prelabelling of human neural precursor cells [J].
Eamegdool, Steven S. ;
Weible, Michael W., II ;
Pham, Binh T. T. ;
Hawkett, Brian S. ;
Grieve, Stuart M. ;
Chan-ling, Tailoi .
BIOMATERIALS, 2014, 35 (21) :5549-5564
1234