NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress

被引：54

作者：

Gavassi, Marina Alves ^{[1
]}

Silva, Giselle Schwab ^{[1
]}

Santiago da Silva, Carolina de Marchi ^{[2
]}

Thompson, Andrew J. ^{[3
]}

Macleod, Kyle ^{[3
]}

Rayner Oliveira, Paulo Marcelo ^{[4
]}

Cavalheiro, Mariana Feitosa ^{[5
]}

Domingues, Douglas Silva ^{[6
]}

Habermann, Gustavo ^{[6
]}

机构：

[1] Univ Estadual Paulista, Inst Biociencias, Dept Biodiversidade, Programa Posgrad Biol Vegetal,UNESP, Av 24-A,1515, BR-13506900 Rio Claro, SP, Brazil

[2] Univ Sao Paulo, ESALQ USP, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Av Padua Dias 11, BR-13418900 Piracicaba, SP, Brazil

[3] Cranfield Univ, Cranfield Soil & Agrifood Inst, Coll Rd, Cranfield MK43 0AL, Beds, England

[4] Univ Sao Paulo, Inst Biocencias, Dept Bot, Rua Matao 14, BR-05508090 Sao Paulo, SP, Brazil

[5] Univ Estadual Campinas, Inst Biol, Ctr Biol Mol & Engn Genet, Programa Posgrad Genet & Biol Mol, Ave Candido Rondon,Cidade Univ, BR-13083875 Campinas, SP, Brazil

[6] Univ Estadual Paulista, Inst Biociencias, Dept Biodiversidade, UNESP, Av 24-A,1515, BR-13506900 Rio Claro, SP, Brazil

来源：

ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 2021年 / 185卷

基金：

巴西圣保罗研究基金会;

关键词：

ABA; Aluminum; Citrus; Plant signalling; Water relations; NCED genes; ABSCISIC-ACID ACCUMULATION; VAPOR-PRESSURE DEFICIT; ROOT CORTICAL-CELLS; 9-CIS-EPOXYCAROTENOID DIOXYGENASE; DIFFERENTIAL EXPRESSION; DROUGHT TOLERANCE; CO2; ASSIMILATION; INDUCED DECREASE; RANGPUR LIME; PLANT;

D O I：

10.1016/j.envexpbot.2021.104404

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (g(s)). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 mu M Al in nutrient solution for 90 days to test whether the low g(s) and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Psi w) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7'-hydroxy-ABA and ABA-beta-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Psi w, RWC and g(s)) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress.

引用

页数：12

共 81 条

[1] Differential expression of putative 9-cis-epoxycarotenoid dioxygenases and abscisic acid accumulation in water stressed vegetative and reproductive tissues of citrus [J].