High prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand

被引：81

作者：

Sasaki, Tadahiro ^{[1
]}

Hirai, Itaru ^{[1
,2
]}

Niki, Marie ^{[1
]}

Nakamura, Tatsuya ^{[3
]}

Komalamisra, Chalit ^{[4
]}

Maipanich, Wanna ^{[4
]}

Kusolsuk, Teera ^{[4
]}

Sa-nguankiat, Surapol ^{[4
]}

Pubampen, Somchit ^{[4
]}

Yamamoto, Yoshimasa ^{[1
,2
]}

机构：

[1] Osaka Univ, Dept Bioinformat, Grad Sch Med, Suita, Osaka 5650871, Japan

[2] Kobe Univ, Grad Sch Med, Kobe, Hyogo 657, Japan

[3] Kansai Med Univ Hosp, Clin Cent Lab, Osaka, Japan

[4] Mahidol Univ, Fac Trop Med, Bangkok, Thailand

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2010年 / 65卷 / 04期

关键词：

genotypes; faecal carriage; rural areas; ESBLs; RESISTANT ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; FECAL CARRIAGE; ANIMALS; SPREAD; HUMANS; FOOD;

D O I：

10.1093/jac/dkq008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

To determine the prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand. Bacteria in stool specimens were screened for extended-spectrum beta-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates. A markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M beta-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M beta-lactamase-producing bacteria were Escherichia coli (85.1%). The study revealed the wide dissemination of CTX-M beta-lactamase-producing Enterobacteriaceae in the healthy population.

引用

页码：666 / 668

页数：3

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