Sensitization of surfactants on the chemiluminescence reaction of fluorescein isothiocyanate labeled proteins

被引：12
作者：
Huang, Chunbao [1 ]
Zhang, Kai
Ci, Yunxiang
机构：
[1] Huanggang Normal Univ, Dept Chem, Huangzhou 438000, Peoples R China
[2] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2007年 / 70卷 / 03期
关键词：
chemiluminescence; sensitization; surfactant; fluorescein isothiocyanate; protein;
D O I：
10.1016/j.jbbm.2006.08.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of surfactants on fluorescein isothiocyanate (FITC)-bovine serum albumin (BSA)-hypochlorite (ClO-), FITC-human serum albumin (HSA)-ClO-, FITC-ovoconalbumin (OVA)-ClO-, FITC-hemoglobin (Hb)-ClO- systems were investigated with chemiluminescence method by the reversed phase flow injection. it was found that the chemiluminescence (CL) intensity of each system was increased greatly in the presence of cationic surfactants. Cethyltrimethylammonium bromide (CTAB) is the optimal surfactant of these systems. The optimal conditions of the CL reaction and the optimal concentration of CTAB were examined and the function of cationic surfactant CTAB on the CL reaction was also discussed. (c) 2006 Elsevier B.V. All rights reserved.
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收藏
页码：341 / 347
页数：7
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