Influence of substratum hydrophobicity on salivary pellicles: organization or composition?

被引:13
作者
Aroonsang, Watcharapong [1 ,2 ]
Sotres, Javier [3 ]
El-Schich, Zahra [3 ]
Arnebrant, Thomas [3 ]
Lindh, Liselott [1 ]
机构
[1] Malmoe Univ, Fac Odontol, Malmo, Sweden
[2] Chiang Mai Univ, Fac Dent, Chiang Mai 50000, Thailand
[3] Malmoe Univ, Fac Hlth & Soc, Malmo, Sweden
关键词
salivary pellicle; SDS-PAGE; immunoblotting; cystatin; amylase; mucin; ACQUIRED ENAMEL PELLICLE; PROLINE-RICH PROTEINS; IN-SITU ELLIPSOMETRY; HUMAN WHOLE SALIVA; FRICTION FORCE SPECTROSCOPY; HYDROXYAPATITE SURFACES; LUBRICATING PROPERTIES; DENTAL MATERIALS; SOLID-SURFACES; ADSORPTION;
D O I
10.1080/08927014.2014.974155
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Different physico-chemical properties (eg adsorption kinetics, thickness, viscoelasticity, and mechanical stability) of adsorbed salivary pellicles depend on different factors, including the properties (eg charge, roughness, wettability, and surface chemistry) of the substratum. Whether these differences in the physico-chemical properties are a result of differences in the composition or in the organization of the pellicles is not known. In this work, the influence of substratum wettability on the composition of the pellicle was studied. For this purpose, pellicles eluted from substrata of different but well-characterized wettabilities were examined by means of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that substratum hydrophobicity did not have a major impact on pellicle composition. In all substrata, the major pellicle components were found to be cystatins, amylases and large glycoproteins, presumably mucins. In turn, interpretation of previously reported data based on the present results suggests that variations in substratum wettability mostly affect the organization of the pellicle components.
引用
收藏
页码:1123 / 1132
页数:10
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