Multi-Residue Determination of Eight Anabolic Steroids by GC-MS in Muscle Tissues from Pigs

A gas chromatography-mass spectrometry (GC-MS) method to determine eight anabolic steroids (diethylstilbestrol, methyltestosterone, norethindrone, 17 alpha-ethynylestradiol, estradiol, 6 alpha-methyl-17 alpha-hydroxy-progesterone, estradiol benzoate, and chlormadinone acetate) was developed. Muscle samples were extracted with liquid-liquid extraction and clean-up was performed in two steps, the extracts obtained were derivatized with heptafluorbutyric (HBF) anhydride and analyzed by GC-MS. In the above method, the linear scope was 2.5-50 mu g kg(-1). The range of the recoveries was 78.5-148% for diethylstilbestrol, 70.8-109% for methyltestosterone, 69.8-87.2% for norethindrone, 67.7-120% for 17 alpha-ethynylestradiol, 82.8-103% for estradiol, 70.3-99.2% for 6 alpha-methyl-17 alpha-hydroxy-progesterone, 73.0-104% for estradiol benzoate, and 72.9-91.8% for chlormadinone acetate. The range of the coefficients of variation within batches was 0.4-12%; the range of the coefficients of variation between batches was 6.4-11%. The limit of detections and the detection capability were 0.99 and 3.30 mu g kg(-1) for diethylstilbestrol, 1.05 and 3.50 mu g kg(-1) for methyltestosterone, 1.19 and 3.97 mu g kg(-1) for norethindrone, 0.94 and 3.13 mu g kg(-)1 for 17 alpha-ethynylestradiol, 1.45 and 4.83 mu g kg(-1) for estradiol, 1.56 and 5.20 mu g kg(-1) for 6 alpha-methyl-17 alpha-hydroxy-progesterone, 1.92 and 6.40 mu g kg(-1) for estradiol benzoate, and 2.41 and 8.03 mu g kg(-1) for chlormadinone acetate, respectively. These results showed that the method was widely available, accurate, and sensitive.