An Engineered Metalloprotein as a Functional and Structural Bioinorganic Model System

被引：12
作者：
Ueno, Takafumi [1 ,2 ]
机构：
[1] Kyoto Univ, Inst Integrated Cell Mat Sci, Nishikyo Ku, Kyoto 6158510, Japan
[2] Kyoto Univ, Funai Ctr, Nishikyo Ku, Kyoto 6158510, Japan
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 23期
关键词：
bioinorganic chemistry; heme proteins; metal coordination; protein engineering; protein structures; NITRIC-OXIDE REDUCTASE; METAL-COMPLEX; ACTIVE-SITE; DESIGN; PROTEIN; MYOGLOBIN; O-2; OXO; NO;
D O I：
10.1002/anie.201000337
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
How best to mimic a protein: Rational design based on the use of a metalloprotein as the molecular scaffold led to a structural (see picture) and functional model of a metalloenzyme that is difficult to obtain in high yield and for which no crystal structure has been determined. This approach will be useful for the preparation of models of sophisticated functional metal centers that are difficult to prepare by chemical synthesis. [Figure Presented] © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：3868 / 3869
页数：2
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