Preserved enzymatic activity of glucose oxidase immobilized on unmodified electrodes for glucose detection

被引:26
作者
Wang, Gang [1 ]
Thai, Ngee Mei [1 ]
Yau, Siu-Tung [1 ]
机构
[1] CUNY Hunter Coll, Dept Phys & Astron, New York, NY 10021 USA
关键词
glucose oxidase; direct electron transfer; biosensor; glucose; HOPG; silicon;
D O I
10.1016/j.bios.2006.10.015
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Glucose sensing electrodes have been realized by immobilizing glucose oxidase (GOx) on unmodified edge plane of highly oriented pyrolytic graphite (epHOPG) and the native oxide of heavily doped silicon (SiO2/Si). Both kinds of electrode show direct interfacial electron transfer due to the redox process of the immobilized GOx. The measured formal potential of the redox process agrees with that of the native enzyme, suggesting that the immobilized GOx has retained its enzymatic activity. The electron transfer rates of the GOx immobilized electrode are 2 s(-1) for GOx/epHOPG electrode and 7.9 s(-1) for GOx/SiO2/Si electrode, which are greater than those for which GOx is immobilized on modified electrodes, probably due to the fact that the enzyme makes direct contact to electrode surface. The preservation of the enzymatic activity of the immobilized GOx has been confirmed by observing the response of the GOx/epHOPG and GCx/SiO2/Si electrodes to glucose with a detection limit of 0.050 mm. The response signals the catalyzed oxidation of glucose and, therefore, confirms that the immobilized GOx retained its enzymatic activity. The properties of the electrode as a glucose sensor are presented. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:2158 / 2164
页数:7
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