Characterisation of eleven new polymorphic microsatellite markers for the coastal stingray Dasyatis brevicaudata (Dasyatidae Hutton 1875), and cross-amplification in seven dasyatid species

被引：3

作者：

Le Port, A. ^{[1
,2
,3
,4
]}

Roycroft, E. J. ^{[2
]}

Thakur, V. ^{[2
]}

Lavery, S. D. ^{[1
,2
]}

机构：

[1] Univ Auckland, Inst Marine Sci, Leigh Marine Lab, POB 349, Warkworth 0941, New Zealand

[2] Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland, New Zealand

[3] James Cook Univ, Ctr Trop Water & Aquat Ecosyst Res TropWATER, Townsville, Qld 4811, Australia

[4] James Cook Univ, Ctr Sustainable Trop Fisheries & Aquaculture, Townsville, Qld 4811, Australia

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2016年 / 65卷

关键词：

Coastal elasmobranch; Microsatellite; Conservation; Habitat degradation; Population genetics; PCR primers; POPULATION-STRUCTURE; PHYLOGEOGRAPHY; SHARK; DNA; MITOCHONDRIAL; SOFTWARE; PRIMERS; PROGRAM; QDD;

D O I：

10.1016/j.bse.2016.02.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Twelve microsatellite loci were developed for the short-tailed stingray Dasyatis brevicaudata using a next-generation sequencing approach, and their utility for population genetics studies was assessed. Eleven out of twelve loci were polymorphic (4-15 alleles) and amplified reliably, with observed heterozygosities between 0.32 and 0.86 (mean = 0.56). Successful cross-species amplification of many of these markers was achieved across seven other dasyatid species currently listed as "Data Deficient" by the IUCN, suggesting these microsatellites will be a useful resource for the investigation of ecological and conservation genetics in a range of species of conservation concern. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：234 / 237

页数：4

共 19 条

[1] Development of microsatellite DNA primers for the giant freshwater stingray, Himantura chaophraya (Batoidea: Dasyatidae) in Thailand, and cross-species amplification in other stingrays [J].

Bhummakasikara, T. ;

Kongrit, C. ;

Siripunkaw, C. ;

Chansue, N. ;

Khudamrongsawat, J. .

CONSERVATION GENETICS RESOURCES, 2013, 5 (02) :453-455

[2] Phylogeography and population structure of thornback rays (Raja clavata L., Rajidae) [J].

Chevolot, Malia ;

Hoarau, Galice ;

Rijnsdorp, Adriaan D. ;

Stam, Wytze T. ;

Olsen, Jeanine L. .

MOLECULAR ECOLOGY, 2006, 15 (12) :3693-3705

[3] Extinction risk and conservation of the world's sharks and rays [J].

Dulvy, Nicholas K. ;

Fowler, Sarah L. ;

Musick, John A. ;

Cavanagh, Rachel D. ;

Kyne, Peter M. ;

Harrison, Lucy R. ;

Carlson, John K. ;

Davidson, Lindsay N. K. ;

Fordham, Sonja V. ;

Francis, Malcolm P. ;

Pollock, Caroline M. ;

Simpfendorfer, Colin A. ;

Burgess, George H. ;

Carpenter, Kent E. ;

Compagno, Leonard J. V. ;

Ebert, David A. ;

Gibson, Claudine ;

Heupel, Michelle R. ;

Livingstone, Suzanne R. ;

Sanciangco, Jonnell C. ;

Stevens, John D. ;

Valenti, Sarah ;

White, William T. .

ELIFE, 2014, 3

[4] Rapid Development of Microsatellite Markers with 454 Pyrosequencing in a Vulnerable Fish, the Mottled Skate, Raja pulchra [J].

Kang, Jung-Ha ;

Park, Jung-Youn ;

Jo, Hyun-Su .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (06) :7199-7211

[5] Phylogeography and conservation of the bull shark (Carcharhinus leucas) inferred from mitochondrial and microsatellite DNA [J].

Karl, S. A. ;

Castro, A. L. F. ;

Lopez, J. A. ;

Charvet, P. ;

Burgess, G. H. .

CONSERVATION GENETICS, 2011, 12 (02) :371-382

[6]

Last P.R., 2009, Sharks and rays of Australia

[7] Speciation of two stingrays with antitropical distributions: low levels of divergence in mitochondrial DNA and morphological characters suggest recent evolution [J].

Le Port, A. ;

Pawley, M. D. M. ;

Lavery, S. D. .

AQUATIC BIOLOGY, 2013, 19 (02) :153-165

[8]

Le Port A, 2009, THESIS U AUCKLAND, P257

[9] Population Structure and Phylogeography of the Short-Tailed Stingray, Dasyatis brevicaudata (Hutton 1875), in the Southern Hemisphere [J].

Le Port, Agnes ;

Lavery, Shane .

JOURNAL OF HEREDITY, 2012, 103 (02) :174-185

[10] QDD version 3.1: a user-friendly computer program for microsatellite selection and primer design revisited: experimental validation of variables determining genotyping success rate [J].

Meglecz, Emese ;

Pech, Nicolas ;

Gilles, Andre ;

Dubut, Vincent ;

Hingamp, Pascal ;

Trilles, Aurelie ;

Grenier, Remi ;

Martin, Jean-Francois .

MOLECULAR ECOLOGY RESOURCES, 2014, 14 (06) :1302-1313

← 1 2 →