O-glycoside orientation is an essential aspect of base J recognition by the kinetoplastid DNA-binding protein JBP1

被引：22
作者：
Grover, Rajesh K.
Pond, Stephanie J. K.
Cui, Qizhi
Subramaniam, Prem
Case, David A.
Millar, David P.
Wentworth, Paul, Jr.
机构：
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[3] Univ Oxford, Dept Biochem, Scripps Oxford Lab, Oxford Glycobiol Inst, Oxford OX1 3QU, England
[4] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[5] Scripps Res Inst Florida, Dept Infectol, Jupiter, FL 32458 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 16期
关键词：
DNA; glycosides; molecular dynamics; molecular recognition;
D O I：
10.1002/anie.200604635
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A binding edge? Comparison of dissociation constants and molecular-dynamics snapshots of a panel of synthetic telomeric double-stranded DNA sequences containing HMdU O-glycosides has revealed that the DNA-binding protein of kinetoplastids, such as Leishmania and Trypanosoma, JBP1, recognizes a critical edge-on conformation of the pyranosyl ring of base J. If this conformation is perturbed, JBP1 binding is dramatically reduced.(Chemical Equation Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2839 / 2843
页数：5
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