The structural characteristic of acidic-hydrolyzed corn silk polysaccharides and its protection on the H2O2-injured intestinal epithelial cells

被引：54
作者：
Jia, Yanan [1 ]
Wang, Yajie [1 ]
Li, Ruilin [1 ]
Li, Shuqin [1 ]
Zhang, Min [2 ,3 ]
He, Chengwei [4 ]
Chen, Haixia [1 ]
机构：
[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China
[2] Tianjin Agr Univ, Tianjin 300384, Peoples R China
[3] Tianjin Univ Sci & Technol, State Key Lab Nutr & Safety, Tianjin 300457, Peoples R China
[4] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China
来源：
FOOD CHEMISTRY | 2021年 / 356卷
关键词：
Corn silk polysaccharides; Acidic-hydrolyzation; Intestinal epithelial cells; Antioxidant activity; ANTIOXIDANT ACTIVITIES; OXIDATIVE STRESS; MOLECULAR-WEIGHT; ELUCIDATION; MECHANISMS; APOPTOSIS; DAMAGE;
D O I：
10.1016/j.foodchem.2021.129691
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This work aims to describe the purification and characterization of acidic-hydrolyzed corn silk polysaccharides (AH-CSP) and evaluate their protection on the H2O2-injured intestinalepithelial cells (IEC-6). Two fractions named AHP-1 and AHP-2 were obtained from AH-CSP, and physicochemical properties of them were investigated by gel permeation chromatography (GPC), gas chromatography (GC), nuclear magnetic resonance (NMR), fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM), and Congo red test. Results showed that AHP-1 (2.80 x 104 Da) and AHP-2 (1.25 x 104 Da) were consisted of xylose, mannose, galactose, rhamnose, arabinose, and glucose. AHP-1 and AHP-2 had strong scavenging activities on 2,2-diphenyl1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and OH. free radicals. Furthermore, pretreatment with AHP-2 could protect the H2O2-injured IEC-6 cells by effectively scavenging the overproduced reactive oxygen species (ROS) and regulating of Kelch-like ECH-associated protein 1(Keap1)/ nuclear factor erythroid 2-related factor 2 (Nrf2) signal pathway.
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页数：9
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