γH2AX as a molecular marker of aging and disease

被引：120
作者：
Mah, Li-Jeen [1 ,2 ]
El-Osta, Assam [1 ,2 ]
Karagiannis, Tom C. [1 ,2 ]
机构：
[1] BakerIDI Heart & Diabet Inst, Alfred Med Res & Educ Precinct, Melbourne, Vic, Australia
[2] Univ Melbourne, Dept Pathol, Parkville, Vic 3052, Australia
来源：
EPIGENETICS | 2010年 / 5卷 / 02期
基金：
英国医学研究理事会;
关键词：
gamma H2AX; double-strand break; chromatin modification; aging; carcinogenesis; DNA damage; repair; DOUBLE-STRAND BREAKS; PHOSPHORYLATED HISTONE H2AX; INFLAMMATORY-BOWEL-DISEASE; DNA-DAMAGE RESPONSE; A-BOMB SURVIVORS; GENOMIC INSTABILITY; IONIZING-RADIATION; OXIDATIVE STRESS; ULCERATIVE-COLITIS; INTERCELLULAR COMMUNICATION;
D O I：
10.4161/epi.5.2.11080
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form gamma H2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete gamma H2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of gamma H2AX as a molecular marker of aging and disease is analysed.
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收藏
页码：129 / 136
页数：8
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