High-affinity ammonium transport by Arabidopsis thaliana AMT1;4

被引：9
|
作者：
Bindel, Nino [1 ]
Neuhauser, Benjamin [1 ]
机构：
[1] Univ Hohenheim, Inst Crop Sci, Nutr Crop Physiol, Fruwirthstr 20, D-70593 Stuttgart, Germany
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2021年 / 43卷 / 04期
关键词：
Ammonium transport; High affinity; AMT; MEP; Electrophysiology;
D O I：
10.1007/s11738-021-03235-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In plants high affinity transport proteins mediate the essential transport of ammonium across membranes. In Arabidopsis thaliana six of these AMmonium Transporters (AMTs) are encoded by the genome. All of them show a unique expression pattern. While most AMTs are highly expressed in the root, AtAMT1;4 expression is limited to the pollen grains and the pollen tube. Here, we addressed the transport characteristics of AtAMT1;4 in the heterologous Xenopus laevis oocytes system. The transport saturated and showed high affinity for ammonium with a K-m value lower than 10 mu M. Based on our electrophysiological analysis, we classified AtAMT1;4 as a high affinity ammonium transporter.
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页数：5
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