High-affinity ammonium transport by Arabidopsis thaliana AMT1;4

被引：9
作者：
Bindel, Nino [1 ]
Neuhauser, Benjamin [1 ]
机构：
[1] Univ Hohenheim, Inst Crop Sci, Nutr Crop Physiol, Fruwirthstr 20, D-70593 Stuttgart, Germany
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2021年 / 43卷 / 04期
关键词：
Ammonium transport; High affinity; AMT; MEP; Electrophysiology;
D O I：
10.1007/s11738-021-03235-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In plants high affinity transport proteins mediate the essential transport of ammonium across membranes. In Arabidopsis thaliana six of these AMmonium Transporters (AMTs) are encoded by the genome. All of them show a unique expression pattern. While most AMTs are highly expressed in the root, AtAMT1;4 expression is limited to the pollen grains and the pollen tube. Here, we addressed the transport characteristics of AtAMT1;4 in the heterologous Xenopus laevis oocytes system. The transport saturated and showed high affinity for ammonium with a K-m value lower than 10 mu M. Based on our electrophysiological analysis, we classified AtAMT1;4 as a high affinity ammonium transporter.
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页数：5
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共 22 条
  • [1] Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily
    Ariz, Idoia
    Boeckstaens, Melanie
    Gouveia, Catarina
    Martins, Ana Paula
    Sanz-Luque, Emanuel
    Fernandez, Emilio
    Soveral, Graca
    von Wiren, Nicolaus
    Marini, Anna M.
    Aparicio-Tejo, Pedro M.
    Cruz, Cristina
    [J]. SCIENCE ADVANCES, 2018, 4 (09):
  • [2] Integrating membrane transport with male gametophyte development and function through transcriptomics
    Bock, KW
    Honys, D
    Ward, JM
    Padmanaban, S
    Nawrocki, EP
    Hirschi, KD
    Twell, D
    Sze, H
    [J]. PLANT PHYSIOLOGY, 2006, 140 (04) : 1151 - 1168
  • [3] Root zone-specific localization of AMTs determines ammonium transport pathways and nitrogen allocation to shoots
    Duan, Fengying
    Giehl, Ricardo F. H.
    Geldner, Niko
    Salta, David E.
    von Wiren, Nicolaus
    [J]. PLOS BIOLOGY, 2018, 16 (10):
  • [4] A twin histidine motif is the core structure for high-affinity substrate selection in plant ammonium transporters
    Ganz, Pascal
    Ijato, Toyosi
    Porras-Murillo, Romano
    Stuehrwohldt, Nils
    Ludewig, Uwe
    Neuhaeuser, Benjamin
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2020, 295 (10) : 3362 - 3370
  • [5] A pore-occluding phenylalanine gate prevents ion slippage through plant ammonium transporters
    Ganz, Pascal
    Mink, Robin
    Ijato, Toyosi
    Porras-Murillo, Romano
    Ludewig, Uwe
    Neuhauser, Benjamin
    [J]. SCIENTIFIC REPORTS, 2019, 9 (1)
  • [6] A Critical Role of AMT2;1 in Root-To-Shoot Translocation of Ammonium in &ITArabidopsis&IT
    Giehl, Ricardo F. H.
    Laginha, Alberto M.
    Duan, Fengying
    Rentsch, Doris
    Yuan, Lixing
    von Wiren, Nicolaus
    [J]. MOLECULAR PLANT, 2017, 10 (11) : 1449 - 1460
  • [7] Substrate binding, deprotonation, and, selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB
    Javelle, Arnaud
    Lupo, Domenico
    Ripoche, Pierre
    Fulford, Tim
    Merrick, Mike
    Winkler, Fritz K.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (13) : 5040 - 5045
  • [8] Mechanism of ammonia transport by Amt/MEP/Rh:: Structure of AmtB at 1.3.5 Å
    Khademi, S
    O'Connell, J
    Remis, J
    Robles-Colmenares, Y
    Miericke, LJW
    Stroud, RM
    [J]. SCIENCE, 2004, 305 (5690) : 1587 - 1594
  • [9] Transport mechanisms in the ammonium transporter family
    Lamoureux, G.
    Javelle, A.
    Baday, S.
    Wang, S.
    Berneche, S.
    [J]. TRANSFUSION CLINIQUE ET BIOLOGIQUE, 2010, 17 (03) : 168 - 175
  • [10] Selective expression of a novel high-affinity transport system for acidic and neutral amino acids in the tapetum cells of Arabidopsis flowers
    Lee, YH
    Tegeder, M
    [J]. PLANT JOURNAL, 2004, 40 (01) : 60 - 74
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