New fluorescent probes for detection and characterization of amyloid fibrils

被引：34

作者：

Gorbenko, Galyna ^{[1
]}

Trusova, Valeriya ^{[1
]}

Kirilova, Elena

Kirilov, Georgiy

Kalnina, Inta

Vasilev, Aleksey ^{[2
]}

Kaloyanova, Stefka ^{[2
]}

Deligeorgiev, Todor ^{[2
]}

机构：

[1] Kharkov Natl Univ, Dept Biol & Med Phys, UA-61077 Kharkov, Ukraine

[2] Univ Sofia, Fac Chem, Dept Appl Organ Chem, Sofia 1164, Bulgaria

来源：

CHEMICAL PHYSICS LETTERS | 2010年 / 495卷 / 4-6期

关键词：

ATOMIC-FORCE MICROSCOPY; ALZHEIMERS-DISEASE; ALPHA-SYNUCLEIN; IMMUNE STATE; THIOFLAVIN-T; PROTEIN; AGGREGATION; INSULIN; REVEAL; AMYLOIDOGENESIS;

D O I：

10.1016/j.cplett.2010.07.005

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The applicability of the novel fluorescent probes, aminoderivative of benzanthrone ABM, squaraine dye SQ-1 and polymethine dye V2 to identification and structural analysis of amyloid fibrils has been evaluated using the lysozyme model system in which fibrillar aggregates have been formed in concentrated ethanol solution. The association constant, binding stoichiometry and molar fluorescence of the bound dye have been determined. ABM was found to surpass classical amyloid marker ThT in the sensitivity to the presence of fibrillar aggregates. Resonance energy transfer measurements involving ABM-SQ-1 and SQ-1-V2 donor-acceptor pairs yielded the limits for fractal-like dimension of lysozyme fibrils. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：275 / 279

页数：5

共 41 条

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