Development of a loop-mediated isothermal amplification method for rapid detection of reticuloendotheliosis virus

被引:12
作者
Deng, Xiaoyun [1 ,2 ]
Qi, Xiaole [1 ]
Gao, Yulong [1 ]
Wang, Yongqiang [1 ]
Qin, Liting [1 ]
Gao, Honglei [1 ]
Gao, Li [1 ]
Wang, Xiaomei [1 ]
机构
[1] Chinese Acad Agr Sci, Div Avian Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 150001, Peoples R China
[2] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China
关键词
Loop-mediated isothermal amplification (LAMP) Reticuloendotheliosis virus (REV); Detection; DISEASE VIRUS; RETROVIRUS INSERTION; IN-VIVO; HERPESVIRUS; PARVOVIRUS;
D O I
10.1016/j.jviromet.2010.04.021
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A loop-mediated isothermal amplification (LAMP) method for rapid detection of reticuloendotheliosis virus (REV) was developed. The method used a set of two pairs of primers to amplify the pot gene for detecting REV, showing high specificity and sensitivity. The REV LAMP method did not cross-react with common avian DNA viruses (Marek's disease virus, chicken anaemia virus, avian leucosis virus of subgroup J). Additionally, the assay could detect different REV strains and had a detection limit of five copies and therefore a higher sensitivity than traditional PCR methods. Furthermore, the efficiency of LAMP for detection REV in clinical samples was comparable to PCR and viral isolation. The procedure of LAMP is simple and does not rely on any special equipment. The detection of REV by LAMP will be useful for detecting and controlling reticuloendotheliosis. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:82 / 86
页数:5
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