Electrospraying microencapsulation of Lactobacillus plantarum enhances cell viability under refrigeration storage and simulated gastric and intestinal fluids

被引：85

作者：

Coghetto, Chaline Caren ^{[1
]}

Brinques, Graziela Brusch ^{[2
]}

Siqueira, Nataly Machado ^{[3
]}

Pletsch, Jessica ^{[3
]}

Duarte Soares, Rosane Michele ^{[3
]}

Zachia Ayub, Marco Antonio ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Food Sci & Technol Inst, Biotechnol & Biochem Engn Lab BiotecLab, BR-91501970 Porto Alegre, RS, Brazil

[2] Fed Univ Hlth Sci Porto Alegre, Dept Nutr, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Inst Chem, Lab Adv Polymers, BR-91501970 Porto Alegre, RS, Brazil

来源：

JOURNAL OF FUNCTIONAL FOODS | 2016年 / 24卷

关键词：

Lactobacillus plantarum; Probiotics; Electrospray; Microencapsulation; Cell viability; PROBIOTIC LACTOBACILLUS; BIFIDOBACTERIUM-LONGUM; ALGINATE BEADS; IN-VITRO; SURVIVAL; ENCAPSULATION; MICROPARTICLES; MICROCAPSULES; PECTIN; GELATION;

D O I：

10.1016/j.jff.2016.03.036

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Electrospraying was used to microencapsulate Lactobacillus plantarum in sodium alginate or in sodium alginate-citric pectin matrixes. Immobilized cells and control (free cells) were tested for survival when exposed to simulated gastric acid and intestinal juices (SGJ and SIJ). Results showed control viability losses of 6 and 4.2 log CFU mL(-1) when exposed for 120 min to SGJ and SIJ, whereas microencapsulated cells decreased by 2.9 CFU mL(-1) in SGJ, and 2.7 CFU mL(-1) in SIJ. Refrigeration storage for 21 days showed cell survivals around 9 log CFU mL(-1) for immobilized cells, whereas for the control the survival, it was around 1 log CFU mL(-1). Microcapsules presented a particle size distribution in the interval of 7 to 2000 gm. FTIR spectra confirmed Ca2+ complexation of alginate and pectin structures obtained by electrospraying. Results demonstrate the efficiency of this technique to increase the survival of L. plantarum in the digestive system of hosts. (c) 2016 Elsevier Ltd. All rights reserved.

引用

页码：316 / 326

页数：11

共 64 条

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