Screening of exopolysaccharide-producing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota

被引:66
作者
Ruas-Madiedo, Patricia [1 ]
Moreno, Jose Antonio [1 ]
Salazar, Nuria [1 ]
Delgado, Susana [1 ]
Mayo, Baltasar [1 ]
Margolles, Abelardo [1 ]
de los Reyes-Gavilan, Clara G. [1 ]
机构
[1] CSIC, Inst Prod Lacteos Asturias, Dept Microbiol Bioquim Prod Lacteos, Villaviciosa 33300, Asturias, Spain
关键词
D O I
10.1128/AEM.02470-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.
引用
收藏
页码:4385 / 4388
页数:4
相关论文
共 21 条
[1]   Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria [J].
Ayad, EHE ;
Nashat, S ;
El-Sadek, N ;
Metwaly, H ;
El-Soda, M .
FOOD MICROBIOLOGY, 2004, 21 (06) :715-725
[2]   Host-bacterial mutualism in the human intestine [J].
Bäckhed, F ;
Ley, RE ;
Sonnenburg, JL ;
Peterson, DA ;
Gordon, JI .
SCIENCE, 2005, 307 (5717) :1915-1920
[3]   Interindividual differences in microbial counts and biochemical-associated variables in the feces of healthy Spanish adults [J].
Delgado, S ;
Ruas-Madiedo, P ;
Suárez, A ;
Mayo, B .
DIGESTIVE DISEASES AND SCIENCES, 2006, 51 (04) :737-743
[4]   Bifidobacterial diversity determined by culturing and by 16S rDNA sequence analysis in feces and mucosa from ten healthy Spanish adults [J].
Delgado, Susana ;
Suarez, Adolfo ;
Mayo, Baltasar .
DIGESTIVE DISEASES AND SCIENCES, 2006, 51 (10) :1878-1885
[5]   Metagenomic analysis of the human distal gut microbiome [J].
Gill, Steven R. ;
Pop, Mihai ;
DeBoy, Robert T. ;
Eckburg, Paul B. ;
Turnbaugh, Peter J. ;
Samuel, Buck S. ;
Gordon, Jeffrey I. ;
Relman, David A. ;
Fraser-Liggett, Claire M. ;
Nelson, Karen E. .
SCIENCE, 2006, 312 (5778) :1355-1359
[6]   Diversity of heteropolysaccharide-producing lactic acid bacterium strains and their biopolymers [J].
Mozzi, Fernanda ;
Vaningelgem, Frederik ;
Hebert, Elvira Maria ;
Van der Meulen, Roel ;
Foulquie Moreno, Maria Remedios ;
Font de Valdez, Graciela ;
De Vuyst, Luc .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (06) :4431-4435
[7]   COMPILATION OF SMALL RIBOSOMAL-SUBUNIT RNA STRUCTURES [J].
NEEFS, JM ;
VANDEPEER, Y ;
DERIJK, P ;
CHAPELLE, S ;
DEWACHTER, R .
NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3025-3049
[8]   Consensus-degenerate hybrid oligonucleotide primers for amplification of priming glycosyltransferase genes of the exopolysaccharide locus in strains of the Lactobacillus casei group [J].
Provencher, C ;
LaPointe, G ;
Sirois, S ;
Van Calsteren, MR ;
Roy, D .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (06) :3299-3307
[9]   Short communication:: Effect of exopolysaccharide isolated from "viili" on the adhesion of probiotics and pathogens to intestinal mucus [J].
Ruas-Madiedo, P. ;
Gueimonde, M. ;
de los Reyes-Gavilan, C. G. ;
Salminen, S. .
JOURNAL OF DAIRY SCIENCE, 2006, 89 (07) :2355-2358
[10]   Exopolysaccharides produced by probiotic strains modify the adhesion of problotics and enteropathogens to human intestinal mucus [J].
Ruas-Madiedo, Patricia ;
Gueimonde, Miguel ;
Margolles, Abelardo ;
Reyes-Gavilan, Clara G. de los ;
Salminen, Seppo .
JOURNAL OF FOOD PROTECTION, 2006, 69 (08) :2011-2015