Screening of exopolysaccharide-producing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota

被引:66
作者
Ruas-Madiedo, Patricia [1 ]
Moreno, Jose Antonio [1 ]
Salazar, Nuria [1 ]
Delgado, Susana [1 ]
Mayo, Baltasar [1 ]
Margolles, Abelardo [1 ]
de los Reyes-Gavilan, Clara G. [1 ]
机构
[1] CSIC, Inst Prod Lacteos Asturias, Dept Microbiol Bioquim Prod Lacteos, Villaviciosa 33300, Asturias, Spain
关键词
D O I
10.1128/AEM.02470-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.
引用
收藏
页码:4385 / 4388
页数:4
相关论文
共 21 条
  • [1] Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria
    Ayad, EHE
    Nashat, S
    El-Sadek, N
    Metwaly, H
    El-Soda, M
    [J]. FOOD MICROBIOLOGY, 2004, 21 (06) : 715 - 725
  • [2] Host-bacterial mutualism in the human intestine
    Bäckhed, F
    Ley, RE
    Sonnenburg, JL
    Peterson, DA
    Gordon, JI
    [J]. SCIENCE, 2005, 307 (5717) : 1915 - 1920
  • [3] Interindividual differences in microbial counts and biochemical-associated variables in the feces of healthy Spanish adults
    Delgado, S
    Ruas-Madiedo, P
    Suárez, A
    Mayo, B
    [J]. DIGESTIVE DISEASES AND SCIENCES, 2006, 51 (04) : 737 - 743
  • [4] Bifidobacterial diversity determined by culturing and by 16S rDNA sequence analysis in feces and mucosa from ten healthy Spanish adults
    Delgado, Susana
    Suarez, Adolfo
    Mayo, Baltasar
    [J]. DIGESTIVE DISEASES AND SCIENCES, 2006, 51 (10) : 1878 - 1885
  • [5] Metagenomic analysis of the human distal gut microbiome
    Gill, Steven R.
    Pop, Mihai
    DeBoy, Robert T.
    Eckburg, Paul B.
    Turnbaugh, Peter J.
    Samuel, Buck S.
    Gordon, Jeffrey I.
    Relman, David A.
    Fraser-Liggett, Claire M.
    Nelson, Karen E.
    [J]. SCIENCE, 2006, 312 (5778) : 1355 - 1359
  • [6] Diversity of heteropolysaccharide-producing lactic acid bacterium strains and their biopolymers
    Mozzi, Fernanda
    Vaningelgem, Frederik
    Hebert, Elvira Maria
    Van der Meulen, Roel
    Foulquie Moreno, Maria Remedios
    Font de Valdez, Graciela
    De Vuyst, Luc
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (06) : 4431 - 4435
  • [7] COMPILATION OF SMALL RIBOSOMAL-SUBUNIT RNA STRUCTURES
    NEEFS, JM
    VANDEPEER, Y
    DERIJK, P
    CHAPELLE, S
    DEWACHTER, R
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (13) : 3025 - 3049
  • [8] Consensus-degenerate hybrid oligonucleotide primers for amplification of priming glycosyltransferase genes of the exopolysaccharide locus in strains of the Lactobacillus casei group
    Provencher, C
    LaPointe, G
    Sirois, S
    Van Calsteren, MR
    Roy, D
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (06) : 3299 - 3307
  • [9] Short communication:: Effect of exopolysaccharide isolated from "viili" on the adhesion of probiotics and pathogens to intestinal mucus
    Ruas-Madiedo, P.
    Gueimonde, M.
    de los Reyes-Gavilan, C. G.
    Salminen, S.
    [J]. JOURNAL OF DAIRY SCIENCE, 2006, 89 (07) : 2355 - 2358
  • [10] Exopolysaccharides produced by probiotic strains modify the adhesion of problotics and enteropathogens to human intestinal mucus
    Ruas-Madiedo, Patricia
    Gueimonde, Miguel
    Margolles, Abelardo
    Reyes-Gavilan, Clara G. de los
    Salminen, Seppo
    [J]. JOURNAL OF FOOD PROTECTION, 2006, 69 (08) : 2011 - 2015