To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

被引：101
作者：
Aguirre, Marisol [1 ,2 ,3 ]
Ramiro-Garcia, Javier [1 ,4 ]
Koenen, Marjorie E. [3 ]
Venema, Koen [1 ,3 ]
机构：
[1] Top Inst Food & Nutr, NL-6700 AA Wageningen, Netherlands
[2] Maastricht Univ, Sch Nutr Toxicol & Metab NUTRIM, Fac Hlth Med & Life Sci, Dept Human Biol, NL-6200 MD Maastricht, Netherlands
[3] Netherlands Org Appl Sci Res, TNO, NL-3700 AJ Zeist, Netherlands
[4] Wageningen Univ, Lab Microbiol Syst & Synthet Biol, NL-6703 HB Wageningen, Netherlands
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 107卷
关键词：
In vitro model; Fecal inoculum preparation; Pooling; Gut microbiota; Fermentation; HUMAN LARGE-INTESTINE; GUT MICROBIOTA; MODEL; BACTERIA; OLIGOSACCHARIDES; METABOLISM; PRODUCTS; PATTERNS; STARCH; CORE;
D O I：
10.1016/j.mimet.2014.08.022
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：1 / 7
页数：7
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