Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro

被引：67

作者：

Chiba, Mitsuru ^{[1
]}

Kubota, Shiori ^{[2
]}

Sato, Konomi ^{[2
]}

Monzen, Satoru ^{[3
]}

机构：

[1] Hirosaki Univ, Grad Sch Hlth Sci, Dept Biosci & Lab Med, 66-1 Hon Cho, Hirosaki, Aomori 0368564, Japan

[2] Hirosaki Univ, Dept Med Technol, Sch Hlth Sci, 66-1 Hon Cho, Hirosaki, Aomori 0368564, Japan

[3] Hirosaki Univ, Grad Sch Hlth Sci, Dept Radiat Sci, 66-1 Hon Cho, Hirosaki, Aomori 0368564, Japan

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

MICROVESICLES; INHIBITION; ACTIVATION; TRIGGERS; RECEPTOR; INDUCE; VEGF;

D O I：

10.1038/s41598-018-30446-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pancreatic cancer has the lowest 5 year survival rate among all cancers. Several extracellular factors are involved in the development and metastasis of pancreatic cancer to distant organs. Exosomes are lipid-bilayer, membrane-enclosed nanoparticles that are recognised as important mediators of cell-to-cell communications. However, the role of exosomes released from pancreatic cancer cells in tumour micro-environment remains unknown. Here, we show that exosomes released from pancreatic cancer PK-45H cells activate various gene expressions in human umbilical vein endothelial cells (HUVECs) by in vitro analyses. In addition, these exosomes released from PK-45H cells promote phosphorylation of Akt and ERK1/2 signalling pathway molecules and tube formation via dynamin-dependent endocytosis in HUVECs. Our findings suggested that exosomes released from pancreatic cancer cells may act as a novel angiogenesis promoter.

引用

页数：9

共 36 条

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