A reliable and stable method for the determination of foretinib in human plasma by LC-MS/MS: Application to metabolic stability investigation and excretion rate

被引:31
作者
Attwa, Mohamed W. [1 ]
Kadi, Adnan A. [1 ]
Darwish, Hany W. [1 ,2 ]
Amer, Sawsan M. [2 ]
Alrabiah, Haitham [1 ]
机构
[1] King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, Riyadh, Saudi Arabia
[2] Cairo Univ, Fac Pharm, Analyt Chem Dept, Cairo, Egypt
关键词
Foretinib; quantification; LC-MS/MS; urine excretion; rat liver microsomes; metabolic stability estimation; TANDEM MASS-SPECTROMETRY; TYROSINE KINASE INHIBITORS; REACTIVE METABOLITES; PHASE-I; RECEPTOR; ELUCIDATION; CANCER;
D O I
10.1177/1469066718768327
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
Foretinib (GSK1363089) is a multiple receptor tyrosine kinases inhibitor. In this study, a reliable, fast liquid chromatography-tandem mass spectrometric method was described for assaying foretinib in plasma, urine, and rat liver microsome samples. Simple extraction procedure by protein preciptation with acetonitrile was implemented for foretinib and brigatinib (internal standard) analysis. Chromatographic resolution of analytes was achieved on C-18 column with the help of isocratic mobile phase. The binary mobile phase consisted of 60% ammonium formate (10 mM, pH 4.2) and 40% acetonitrile at a flow rate of 0.25 mL/min. Run time was 3 min, and both foretinib and brigatinib were eluted within 0.74 and 1.95 min; they were detected in positive ion mode utilizing multiple reactions monitoring mode. Linearity of the proposed method ranged from 5 to 500 ng/mL (r(2) >= 0.9993) in the human plasma. Lower limit of quantification and detection were 6.0 and 1.8 ng/mL, respectively. Intraday and interday precision and accuracy were 0.16 to 1.67 % and -2.39 to -0.52 %. In vitro half-life and intrinsic clearance were 24.93 min and 6.56 mL/min/kg, respectively. Literature review showed that no previous studies have been proposed for the analytical quantification of foretinib in human plasma or its metabolic stability. The established method was also applied to estimate the rate of foretinib excretion in rat urine. The developed method can be used for foretinib pharmacokinetic applications.
引用
收藏
页码:344 / 351
页数:8
相关论文
共 21 条
[1]   LC-MS/MS method for the quantification of masitinib in RLMs matrix and rat urine: application to metabolic stability and excretion rate [J].
Amer, Sawsan M. ;
Kadi, Adnan A. ;
Darwish, Hany W. ;
Attwa, Mohamed W. .
CHEMISTRY CENTRAL JOURNAL, 2017, 11
[2]   Liquid chromatography tandem mass spectrometry method for the quantification of vandetanib in human plasma and rat liver microsomes matrices: metabolic stability investigation [J].
Amer, Sawsan M. ;
Kadi, Adnan A. ;
Darwish, Hany W. ;
Attwa, Mohamed W. .
CHEMISTRY CENTRAL JOURNAL, 2017, 11
[3]   Identification and characterization of in vitro phase I and reactive metabolites of masitinib using a LC-MS/MS method: bioactivation pathway elucidation [J].
Amer, Sawsan M. ;
Kadi, Adnan A. ;
Darwish, Hany W. ;
Attwa, Mohamed W. .
RSC ADVANCES, 2017, 7 (08) :4479-4491
[4]  
Baranczewski P, 2006, PHARMACOL REP, V58, P453
[5]   Investigation of metabolic stability of the novel ALK inhibitor brigatinib by Liquid chromatography tandem mass spectrometry [J].
Darwish, Hany W. ;
Kadi, Adnan A. ;
Attwa, Mohamed W. ;
Almutairi, Halah S. .
CLINICA CHIMICA ACTA, 2018, 480 :180-185
[6]   A Phase I Study of Foretinib, a Multi-Targeted Inhibitor of c-Met and Vascular Endothelial Growth Factor Receptor 2 [J].
Eder, Joseph Paul ;
Shapiro, Geoffrey I. ;
Appleman, Leonard J. ;
Zhu, Andrew X. ;
Miles, Dale ;
Keer, Harold ;
Cancilla, Belinda ;
Chu, Felix ;
Hitchcock-Bryan, Suzanne ;
Sherman, Laurie ;
McCallum, Stewart ;
Heath, Elisabeth I. ;
Boerner, Scott A. ;
LoRusso, Patricia M. .
CLINICAL CANCER RESEARCH, 2010, 16 (13) :3507-3516
[7]   The AXL receptor tyrosine kinase is associated with adverse prognosis and distant metastasis in esophageal squamous cell carcinoma [J].
Hsieh, Min-Shu ;
Yang, Pei-Wen ;
Wong, Li-Fan ;
Lee, Jang-Ming .
ONCOTARGET, 2016, 7 (24) :36956-36970
[8]   Validated LC-MS/MS Method for the Quantification of Ponatinib in Plasma: Application to Metabolic Stability [J].
Kadi, Adnan A. ;
Darwish, Hany W. ;
Attwa, Mohamed W. ;
Amer, Sawsan M. .
PLOS ONE, 2016, 11 (10)
[9]   Detection and characterization of ponatinib reactive metabolites by liquid chromatography tandem mass spectrometry and elucidation of bioactivation pathways [J].
Kadi, Adnan A. ;
Darwish, Hany W. ;
Attwa, Mohamed W. ;
Amer, Sawsan M. .
RSC ADVANCES, 2016, 6 (76) :72575-72585
[10]   A highly efficient and sensitive LC-MS/MS method for the determination of afatinib in human plasma: application to a metabolic stability study [J].
Kadi, Adnan A. ;
Abdelhameed, Ali S. ;
Darwish, Hany W. ;
Attwa, Mohamed W. ;
Al-Shakliah, Nasser S. .
BIOMEDICAL CHROMATOGRAPHY, 2016, 30 (08) :1248-1255